DDX42 Antibody

Datasheet

Code	CSB-PA773053LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA773053LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA773053LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells cells with CSB-PA773053LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) DDX42 Polyclonal antibody

Uniprot No.

Q86XP3

Target Names

DDX42

Alternative Names

DDX42ATP-dependent RNA helicase DDX42 antibody; EC 3.6.4.13 antibody; DEAD box protein 42 antibody; RNA helicase-like protein antibody; RHELP antibody; RNA helicase-related protein antibody; RNAHP antibody; SF3b DEAD box protein antibody; Splicing factor 3B-associated 125 kDa protein antibody; SF3b125 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human ATP-dependent RNA helicase DDX42 protein (519-819AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The DDX42 Antibody (Product code: CSB-PA773053LA01HU) is Non-conjugated. For DDX42 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA773053LB01HU	DDX42 Antibody, HRP conjugated	ELISA
FITC	CSB-PA773053LC01HU	DDX42 Antibody, FITC conjugated
Biotin	CSB-PA773053LD01HU	DDX42 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.

Gene References into Functions

characterization of protein associated with the human 17S U2 snRNP and one of its stable subunits, SF3b; may facilitate the assembly of the 17S U2 snRNP PMID: 12234937
Ddx42p represents the first example of a human DEAD box protein possessing RNA helicase, protein displacement and RNA annealing activities. PMID: 16397294
expression of N-terminal DDX42 is able to overcome Japanese encephalitis virus-induced antagonism of IFN responses and so may be important for Japanese encephalitis virus pathogenesis PMID: 18588927

Subcellular Location

Cytoplasm. Nucleus speckle. Nucleus, Cajal body. Note=Isoform 2 is present in Cajal bodies (CBs) and nuclear speckles.

Protein Families

DEAD box helicase family, DDX42 subfamily

Tissue Specificity

Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.

Database Links

HGNC: 18676

OMIM: 613369

KEGG: hsa:11325

STRING: 9606.ENSP00000374574

UniGene: Hs.702010

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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