Product Details

Uniprot No.

Target Names

IFIT3

Alternative Names

CIG 49 antibody; CIG49 antibody; GARG 49 antibody; IFI-60K antibody; IFI60 antibody; IFI60K antibody; IFIT-3 antibody; IFIT-4 antibody; Ifit3 antibody; IFIT3_HUMAN antibody; IFIT4 antibody; Interferon induced 60 kDa protein antibody; Interferon induced protein 60 antibody; Interferon induced protein with tetratricopeptide repeats 3 antibody; Interferon-induced 60 kDa protein antibody; Interferon-induced protein with tetratricopeptide repeats 3 antibody; Interferon-induced protein with tetratricopeptide repeats 4 antibody; IRG2 antibody; ISG-60 antibody; ISG60 antibody; P60 antibody; Retinoic acid induced gene G protein antibody; Retinoic acid-induced gene G protein antibody; RIG G antibody; RIG-G antibody; RIGG antibody; RP11-149I23.4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Human IFIT3

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

IFN-induced antiviral protein which acts as an inhibitor of cellular as well as viral processes, cell migration, proliferation, signaling, and viral replication. Enhances MAVS-mediated host antiviral responses by serving as an adapter bridging TBK1 to MAVS which leads to the activation of TBK1 and phosphorylation of IRF3 and phosphorylated IRF3 translocates into nucleus to promote antiviral gene transcription. Exhibits an antiproliferative activity via the up-regulation of cell cycle negative regulators CDKN1A/p21 and CDKN1B/p27. Normally, CDKN1B/p27 turnover is regulated by COPS5, which binds CDKN1B/p27 in the nucleus and exports it to the cytoplasm for ubiquitin-dependent degradation. IFIT3 sequesters COPS5 in the cytoplasm, thereby increasing nuclear CDKN1B/p27 protein levels. Upregulates CDKN1A/p21 by downregulating MYC, a repressor of CDKN1A/p21. Can negatively regulate the apoptotic effects of IFIT2.

Gene References into Functions

We provided the evidence that IFIT3 was up-regulated during hepatic ischemia-reperfusion injury (IRI) and knockdown of IFIT3 exerted potent protective effects against hepatic IRI in vitro and in vivo. Our findings not only revealed the mechanism for IFIT3-regulated hepatic IRI, but also proposed potential clinical significance of treatment targeting IFIT3 for patients undergoing liver resection and liver transplantation. PMID: 29734133
These findings indicated that hepatitis B virus-induced miR146a attenuates cell-intrinsic anti-viral innate immunity through targeting RIG-I and RIG-G. PMID: 27210312
Low RIG-G expression is associated with lung cancer. PMID: 27602766
Biomarker expression in pancreatic ductal adenocarcinoma (PDAC) of CXCR4, SMAD4, SOX9 and IFIT3 will be prospectively assessed by immunohistochemistry and verified by rt.-PCR from tumor and adjacent healthy pancreatic tissue of surgical specimen. PMID: 28356064
these data suggest that postpartum, the normalization of the physiological rheostat controlling IFN signaling depends on IFNL3 genotype. PMID: 27601663
Higher expression of IFIT3 enhances anti-apoptotic activity and chemotherapy resistance of pancreatic ductal adenocarcinoma cells. High expression of IFIT3 was independently correlated to shorter patients' survival and may serve as a prognostic marker. PMID: 28210844
Among the associated variants were two in regions previously unreported for COPD; a low frequency non-synonymous SNP in MOCS3 (rs7269297, pdiscovery=3.08x10(-6), preplication=0.019) and a rare SNP in IFIT3, which emerged in the meta-analysis (rs140549288, pmeta=8.56x10(-6)). PMID: 26917578
HSV-1 was shown for the first time to evade the antiviral function of IFIT3 via UL41. PMID: 27681138
results indicated that RIG-G level was high in maturated cells and low in blast cells, and suggested that RIG-G might play a role in the differentiation of bone marrow hemocytes in vivo PMID: 26686474
The transcription factor SOX9, which is linked to regulation of hypoxia-related genes, was identified as a key mediator of upregulation of the oncogene IFIT3 and thereby sustaining a "pseudoinflammatory" cellular condition in pancreatic tumors. PMID: 25650658
Reovirus T3D infection induced STAT-1, ISG-15, IFIT-1, Mx1 and IFIT-3 expression. PMID: 25905045
In cell models of dengue virus 2 infection, authors found that IFITM3 contributed to both the baseline and interferon-induced inhibition of virus entry. PMID: 25131332
protective roles of IFIT3 following IFN-alpha production in DV infection of human lung epithelial cells PMID: 24223959
these findings reveal for the first time the negative regulation of Rig-G on SCF-E3 ligase activities through disrupting CSN complex, not only contributing to further investigation on biological functions of Rig-G, but also leading to better understanding of the CSN complex as a potential target in tumor diagnosis and treatment. PMID: 23415865
RIG-G gene expression is closely correlated with the cross-talk between all-trans retinoic acid and IFN-alpha-induced signaling pathways in NB4 tumor cells. PMID: 22490698
our study characterizes IFIT3 as an important modulator in innate immunity PMID: 21813773
All-trans retinoic acid upregulated RIG-G in NB4 cells by upregulating IRF1, IRF9 and STAT2. PMID: 20137113
The complex STAT2/IRF-9 is the key factor mediating the expression of RIG-G gene regulated by IFN-alpha. PMID: 20403236
concluded that the expression of RIG-G was independent on the classical JAK-STAT pathway, but could be greatly increased by it. PMID: 21056555
Identification of alpha interferon-induced genes associated with antiviral activity in Daudi cells and characterization of IFIT3 as a novel antiviral gene. PMID: 20686046
transcription factors for the reporter gene. CONCLUSION: Both ISRE I and ISRE II on the RIG-G promoter are the binding sites for the complex of transcription factors. They are required for RIG-G expression, and ISRE I has a preferential role over ISRE II. PMID: 20533260
The induction of IFIT4 transcription by IFN-alpha depends upon sequential activation of PKCdelta, JNK and STAT1, and the influence of PKCdelta or JNK on IFN-alpha-mediated induction of IFIT4 is dependent upon the phosphorylation of STAT1 at Ser-727. PMID: 17933493
IFIT4 may play roles in promoting monocyte differentiation into dendritic cell-like cells (DCLCs) and in directing DCLCs to modulate T-helper-1 cell differentiation, thus contributing to the autoimmunity and pathogenesis of systemic lupus erythematosus. PMID: 18706081
Induction of RIG-G by retinoic acid in NB4 cells resulted, to some extent, from an IFNalpha autocrine pathway, a finding that suggests a novel mechanism for the signal cross-talk between IFNalpha and retinoic acid. PMID: 19351818

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Subcellular Location

Cytoplasm. Mitochondrion.

Protein Families

IFIT family

Tissue Specificity

Expression significantly higher in peripheral blood mononuclear cells (PBMCs) and monocytes from systemic lupus erythematosus (SLE) patients than in those from healthy individuals (at protein level). Spleen, lung, leukocytes, lymph nodes, placenta, bone m

Database Links

HGNC: 5411

OMIM: 604650

KEGG: hsa:3437

STRING: 9606.ENSP00000360876

UniGene: Hs.47338