MED28 Antibody

Datasheet

Code	CSB-PA863955LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA863955LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA863955LA01HU at dilution of 1:100
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) MED28 Polyclonal antibody

Uniprot No.

Q9H204

Target Names

MED28

Alternative Names

EG1 antibody; Endothelial-derived protein 1 antibody; FKSG20 antibody; Magicin antibody; med28 antibody; MED28_HUMAN antibody; Mediator complex subunit 28 antibody; Mediator of RNA polymerase II transcription subunit 28 antibody; Merlin and Grb2-interacting cytoskeletal protein antibody; Tumor angiogenesis marker EG-1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Mediator of RNA polymerase II transcription subunit 28 protein (1-178AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The MED28 Antibody (Product code: CSB-PA863955LA01HU) is Non-conjugated. For MED28 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863955LB01HU	MED28 Antibody, HRP conjugated	ELISA
FITC	CSB-PA863955LC01HU	MED28 Antibody, FITC conjugated
Biotin	CSB-PA863955LD01HU	MED28 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

Gene References into Functions

MED28 modulates the development of epithelial-mesenchymal transition through NFkappaB in human breast cancer cells. PMID: 27662245
All trans-retinoic acid can reverse the suppressive effect of MED28 on HBP1 and E-cadherin and inactivate the Wnt/beta-catenin pathway in colorectal cancer, suggesting a protective effect of ATRA against colorectal cancer. PMID: 26660958
This paper describes a link between Med23 and IFN-lambda3, provides evidence for the crucial role of IFN-lambda in HSV-1 immune control. PMID: 23950709
These data indicate that MED28 regulates cellular migration in a MEK1-dependent manner in human breast cancer cells, reinforcing the important cellular roles of MED28. PMID: 22495818
High MED28 is associated with breast cancer. PMID: 20584319
demonstrate an interaction between Grb2 and magicin. In addition, merlin is capable of forming a ternary complex with magicin and Grb2 PMID: 15467741
EG-1 is a positive stimulator of cellular proliferation, and may possibly be involved in signaling pathways involving Src and MAPK activation. PMID: 16024617
May serve as a multi-faceted adaptor/scaffold to relay cellular signaling to the cytoskeleton and from the cytoskeleton to the nucleus. PMID: 16899217
EG-1 may be involved in signaling pathways including c-Src activation. PMID: 16964398
EG-1 has a role in breast tumor growth PMID: 17568184
Study reports that down-regulation of human Med28 expression in NIH3T3 cells results in a significant induction of several genes associated with smooth muscle cell (SMC)differentiation; conversely, overexpression of MED28 represses SMC gene expression. PMID: 17848560

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Subcellular Location

Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.

Protein Families

Mediator complex subunit 28 family

Tissue Specificity

Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.

Database Links

HGNC: 24628

OMIM: 610311

KEGG: hsa:80306

STRING: 9606.ENSP00000237380

UniGene: Hs.731966

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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