POMP Antibody

Datasheet

Code	CSB-PA018365LA01HU
Size	US$166
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Image	IHC image of CSB-PA018365LA01HU diluted at 1:400 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA018365LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) POMP Polyclonal antibody

Uniprot No.

Q9Y244

Target Names

POMP

Alternative Names

2510048O06Rik antibody; C13orf12 antibody; Chromosome 13 open reading frame 12 antibody; HSPC 014 antibody; HSPC036 protein antibody; hUMP 1 antibody; hUMP1 antibody; PNAS 110 antibody; PNAS110 antibody; Pomp antibody; POMP_HUMAN antibody; Proteasome maturation protein antibody; Proteassemblin antibody; Protein UMP1 homolog antibody; UMP 1 antibody; UMP1 antibody; UMP1; yeast; homolog of antibody; Voltage gated K channel beta subunit 4.1 antibody; Voltage-gated K channel beta subunit 4.1 antibody; voltage-gated potassium channel beta subunit 4.1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Proteasome maturation protein (1-141AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The POMP Antibody (Product code: CSB-PA018365LA01HU) is Non-conjugated. For POMP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018365LB01HU	POMP Antibody, HRP conjugated	ELISA
FITC	CSB-PA018365LC01HU	POMP Antibody, FITC conjugated
Biotin	CSB-PA018365LD01HU	POMP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.

Gene References into Functions

POMP-related autoinflammation and immune dysregulation disease (PRAID) discovered in two unrelated individuals with a unique constellation of early-onset combined immunodeficiency, inflammatory neutrophilic dermatosis, and autoimmunity, is reported. PMID: 29805043
POMP is overexpressed and participates in the increase of proteasome assembly and activity in psoriatic skin. PMID: 28728908
MCM3AP and POMP Mutations Cause a DNA-Repair and DNA-Damage-Signaling Defect in an Immunodeficient Child PMID: 26615982
studies validate a role for the NRF2/POMP axis in bortezomib resistance and identify NRF2 and POMP as potentially attractive targets for chemosensitization to this proteasome inhibitor PMID: 26483548
microRNA-101 (miR-101) targets the proteasome maturation protein POMP, leading to impaired proteasome assembly and activity, and resulting in accumulation of p53 and cyclin-dependent kinase inhibitors, cell cycle arrest, and apoptosis. PMID: 26145175
Single-nucleotide polymorphisms in POMP gene is associated with breast cancer risk after menopausal hormone replacement therapy. PMID: 24080446
KLICK is caused by reduced levels of POMP, leading to proteasome insufficiency in differentiating keratinocytes PMID: 22235297
A single-nucleotide deletion in the POMP 5' UTR causes a transcriptional switch and altered epidermal proteasome distribution in KLICK genodermatosis. PMID: 20226437
POMP/proteassemblin/hUmp1 may be tetramerised and is localized to the cytoplasm and the nucleus PMID: 16624403
Data show that POMP facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. PMID: 17948026

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Involvement in disease

Keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK)

Subcellular Location

Cytoplasm, cytosol. Nucleus. Microsome membrane.

Protein Families

POMP/UMP1 family

Tissue Specificity

Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer.

Database Links

HGNC: 20330

OMIM: 601952

KEGG: hsa:51371

STRING: 9606.ENSP00000370222

UniGene: Hs.268742

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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