VIPAS39 Antibody

Datasheet

Code	CSB-PA888005LA01HU
Size	US$166
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Image	Western blot All lanes: VIPAS39 antibody at 12µg/ml Lane 1: HepG2 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Mouse kidney tissue Lane 5: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58, 52 kDa Observed band size: 58 kDa Immunofluorescence staining of Hela cells with CSB-PA888005LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VIPAS39 Polyclonal antibody

Uniprot No.

Q9H9C1

Target Names

VIPAS39

Alternative Names

Apical basolateral polarity regulator antibody; FLJ12707 antibody; hSPE-39 antibody; Protein spe-39 homolog antibody; SPE 39 antibody; SPE 39 protein antibody; SPE39 antibody; Uncharacterized protein C14orf133 antibody; vipar antibody; VIPAR_HUMAN antibody; VPS16B antibody; VPS33B interacting protein antibody; VPS33B interacting protein apical basolateral polarity regulator antibody; VPS33B interacting protein involved in polarity and apical protein restriction antibody; VPS33B-interacting protein antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Spermatogenesis-defective protein 39 homolog protein (1-493AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The VIPAS39 Antibody (Product code: CSB-PA888005LA01HU) is Non-conjugated. For VIPAS39 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA888005LB01HU	VIPAS39 Antibody, HRP conjugated	ELISA
FITC	CSB-PA888005LC01HU	VIPAS39 Antibody, FITC conjugated
Biotin	CSB-PA888005LD01HU	VIPAS39 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity. May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be independent of VPS33B. May play a role in vesicular trafficking during spermatogenesis. May be involved in direct or indirect transcriptional regulation of E-cadherin.

Gene References into Functions

A likely causal mutation was identified in the majority (61%), spanning many genes including ones that have only rarely been reported to cause cholestatic liver disease, e.g. TJP2 and VIPAS39 PMID: 28039895
Genetic studies showed a homozygous mutation in the VIPAS39 gene. Making the definite diagnosis of the syndrome is important, while increased risk of mutation in other siblings highlights the importance of prenatal diagnosis. PMID: 26808426
Our data suggest that the ARC syndrome may result through impaired VIPAS39/SPE-39 and Vps33b-dependent endosomal maturation or fusion. PMID: 23918659
VPS16B, similar to its binding partner VPS33B, is essential for megakaryocyte and platelet alpha-granule biogenesis. PMID: 23002115
SPE-39 has an inhibitory effect due to tyrosine phosphorylation and ubiquitination on the function of Vps33B in the EGF-stimulated cells PMID: 22677173
SPE-39 homologues are present in RAB5-, RAB7-, and RAB11-positive endosomes where they play a conserved role in lysosomal delivery and probably function via their interaction with the core HOPS complex. PMID: 19109425

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Involvement in disease

Arthrogryposis, renal dysfunction and cholestasis syndrome 2 (ARCS2)

Subcellular Location

Cytoplasm. Cytoplasmic vesicle. Early endosome. Recycling endosome. Late endosome. Note=Colocalizes in clusters with VPS33B at cytoplasmic organelles (PubMed:19109425).

Protein Families

SPE39 family

Database Links

HGNC: 20347

OMIM: 613401

KEGG: hsa:63894

STRING: 9606.ENSP00000339122

UniGene: Hs.16157

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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