Kanamycin Monoclonal Antibody

Datasheet

Code	CSB-MA000511I0m
Size	US$210
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Product Details
Citations
Customer Reviews and Q&A

Product Details

Target Names

Alternative Names

Amkin, biograncetin, enterokan

Raised in

mouse

Immunogen

Kanamycin-BSA conjugate

Conjugate

Non-conjugated

Clonality

monoclonal

Isotype

IgG1

Purification Method

>95%，protein G purifed

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

liquid

Tested Applications

For research use only

Intended Use

ELISA, Immune colloidal gold, Immunochromatography

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The mice spleens isolated from kanamycin-BSA-immunized mice are fused with myeloma cells to generate hybridomas. Hybridoma cells are further injected into the abdominal cavity of mice, producing and obtaining mouse ascites fluid containing kanamycin monoclonal antibody. This monoclonal antibody occurs as an unconjugated IgG1. Its purity is greater than 95% using protein G purified. The target kanamycin is an aminoglycoside antibiotic used to treat severe bacterial infections and tuberculosis. And this anti-kanamycin monoclonal antibody has been validated for use in the ELISA assay.

Citations

A Novel Method for Antibiotic Detection in Milk Based on Competitive Magnetic Immunodetection Jan Pietschmann,Foods (Basel, Switzerland),2020

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Determination of Antibody Affinity

Review: For testing the affinity of corresponding mAb towards kanamycin-BSA conjugate in an ELISA, determination of antibody affinity was performed. It suggested that high affinity of respective antibody against their antigen-conjugate could be detected with EC50-values of 177.7 ng·mL-1 for kanamycin-specific mAb. Absorbance was measured after 10 min of ABTS substrate incubation. n = 1.

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