Recombinant Rat Small conductance calcium-activated potassium channel protein 2 (Kcnn2)

Code CSB-CF012084RA
Size $1620
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Kcnn2
Uniprot No.
Research Area
Neuroscience
Species
Rattus norvegicus (Rat)
Source
in vitro E.coli expression system
Expression Region
1-580aa
Target Protein Sequence
MSSCRYNGGVMRPLSNLSSSRRNLHEMDSEAQPLQPPASVVGGGGGASSPSAAAAASSSAPEIVVSKPEHNNSNNLALYGTGGGGSTGGGGGGGGGGGGSGHGSSSGTKSSKKKNQNIGYKLGHRRALFEKRKRLSDYALIFGMFGIVVMVIETELSWGAYDKASLYSLALKCLISLSTIILLGLIIVYHAREIQLFMVDNGADDWRIAMTYERIFFICLEILVCAIHPIPGNYTFTWTARLAFSYAPSTTTADVDIILSIPMFLRLYLIARVMLLHSKLFTDASSRSIGALNKINFNTRFVMKTLMTICPGTVLLVFSISLWIIAAWTVRACERYHDQQDVTSNFLGAMWLISITFLSIGYGDMVPNTYCGKGVCLLTGIMGAGCTALVVAVVARKLELTKAEKHVHNFMMDTQLTKRVKNAAANVLRETWLIYKNTKLVKKIDHAKVRKHQRKFLQAIHQLRSVKMEQRKLNDQANTLVDLAKTQNIMYDMISDLNERSEDFEKRIVTLETKLETLIGSIHALPGLISQTIRQQQRDFIETQMENYDKHVTYNAERSRSSSRRRRSSSTAPPTSSESS-
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
69.9 kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization.
Gene References into Functions
  1. Study characterized and genetically analyzed F344-Trdk/+ rats. Tremor behaviors were suppressed by anti-tremor agents for essential tremor, but not for Parkinson's disease. A missense mutation, I289N, was detected in the Kcnn2 gene of F344-Trdk/+ rats. Electrophysiological studies in HEK293 cells revealed that this mutation diminished KCNN2 channel function. PMID: 28917524
  2. SK2 channels regulate mitochondrial respiration and mitochondrial Ca(2+) uptake. PMID: 28282037
  3. EDH-mediated responses are impaired in superior mesenteric arteries of SHRSP because of a reduction in both TRPV4 and SKCa input to EDH. PMID: 27872234
  4. Apamin-Sensitive K+ Current Upregulation in Volume-Overload Heart Failure is Associated with the Decreased Interaction of CK2 with SK2. PMID: 26362340
  5. Sarcoplasmic reticulum Ca(2+) release is both necessary and sufficient for the activation of SK channels in rat ventricular myocytes. PMID: 24381116
  6. SK2 and SK3 channels are expressed in different populations of motoneurons in rats and mice but not in cats PMID: 23129791
  7. SK2 channels are expressed in rat cerebellar Purkinje cells during development and throughout adulthood PMID: 21521760
  8. Allosteric block of KCa2 channels by apamin. PMID: 20562108
  9. SK2 is expressed in inner hair cells during postnatal weeks 1-2. It is downregulated after the onset of hearing. PMID: 15356192
  10. In the intact SK channel complex, the N-lobe of calmodulin provides ligand-binding sites for channel gating, and that its ligand-binding properties are comparable to those of the N-lobe in isolated calmodulin. PMID: 19144926

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Potassium channel KCNN family, KCa2.2/KCNN2 subfamily
Tissue Specificity
Brain.
Database Links
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