Recombinant Rat BRAK protein (Cxcl14)

CXCL14 (BRAK) is a homeostatic chemokine with distinctive signaling properties, functioning as a potent chemoattractant for monocytes and dendritic cells while lacking activity on neutrophils — making it a valuable tool for dissecting selective immune cell recruitment. This tag-free recombinant rat CXCL14 encompasses the full-length mature sequence (residues 23–99) and demonstrates robust chemotactic activity on human monocytes at 1.0–10 ng/ml, providing a suitable basis for Transwell migration assays, monocyte activation studies, and downstream signaling investigations involving MAPK and NF-κB pathways. The endotoxin level of less than 1.0 EU/μg satisfies the stringent criteria typical for primary immune cell assays, ensuring that observed chemotactic or activation responses reflect genuine CXCL14 signaling rather than LPS-driven artifacts. Purity exceeding 95% by SDS-PAGE further supports use as a reference standard in cytokine detection assays, a positive control for antibody validation, or a defined stimulus in rat inflammation models.