GRF6 Antibody

Code CSB-PA343727XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) GRF6 Polyclonal antibody
Uniprot No.
Target Names
GRF6
Alternative Names
GRF6 antibody; AFT1 antibody; RCI2 antibody; At5g10450 antibody; F12B17_20014-3-3-like protein GF14 lambda antibody; 14-3-3-like protein AFT1 antibody; 14-3-3-like protein RCI2 antibody; General regulatory factor 6 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana GRF6 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes. Specific negative regulator of slow-vacuolar (SV) ion channel. Mediates F-actin dynamics possibly through inhibiting ADF1 phosphorylation. Negative regulator of freezing tolerance that modulates cold-responsive C-repeat-binding factors (CBF) DREB1A AND DREB1B proteins stability by facilitating their ubiquitin-mediated degradation when activated by CRPK1-mediated phosophorylation in freezing conditions.
Gene References into Functions
  1. The 14-3-3 lambda mutation impairs stomatal opening mediated by PHOT2. PMID: 22408078
  2. study suggest that GF14lambda may positively regulate the RPW8.2 resistance function and play a role in enhancing basal resistance in Arabidopsis. PMID: 19624472
  3. findings show that AtTPK1 (KCO1)interacts with 14-3-3 proteins (general regulating factors, GRFs); TPK1 and GRF6 co-localize at the vacuolar membrane PMID: 17764516
  4. the 14-3-3 lambda (lambda) isoform from Arabidopsis. 14-3-3lambda and phot1 were found to colocalise and interact in vivo PMID: 19524572
Subcellular Location
Nucleus. Cell membrane. Cytoplasm.
Protein Families
14-3-3 family
Database Links

KEGG: ath:AT5G10450

STRING: 3702.AT5G10450.4

UniGene: At.24269

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