Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) B'GAMMA Polyclonal antibody

Uniprot No.

Q8RW96

Target Names

B'GAMMA

Alternative Names

B'GAMMA antibody; At4g15415 antibody; dl3750w antibody; FCAALL.118Serine/threonine protein phosphatase 2A 59 kDa regulatory subunit B' gamma isoform antibody; AtB' gamma antibody; PP2A antibody; B' subunit antibody; gamma isoform antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana B'GAMMA protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

The B regulatory subunit may modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment. Required for the formation of the PP2A holoenzyme that negatively regulates brassinosteroid signaling by dephosphorylating and inactivating BRI1 in the cytoplasm. Seems to be functionally connected with CPR5 and may mediate the negative regulation of defense reactions and senescence under low irradiances. May contribute to the epigenetic regulation of defense gene expression. Involved in the control of methoxylation of indole glucosinolates and formation of 4-methoxy- indol-3-yl-methyl glucosinolate in leaves, through direct interaction with indole glucosinolate methyltransferases. Involved in growth regulation and stress signaling. Involved in the regulation of reactive oxygen species (ROS) signaling and maintenance of cellular ROS homeostasis. Required to control the level of ACO3 phosphorylation in the cytoplasm. Regulates hydrogen peroxide metabolism by controlling the abundance of AOX1A and AXO3/AOX1D in leaf mitochondria. May mediate dephosphorylation of CRT1 and promote the degradation of unfolded proteins in endoplasmic reticulum (ER). Involved in the regulation of flowering time by repressing FLC, the main flowering repressor gene.

Gene References into Functions

Data show that the cytoplasmic protein phosphatase 2A regulatory subunit B'gamma (PP2A-B'gamma) is required to control the amount of a specifically methoxylated indole glucosinolate 4MO-I3M. PMID: 27598402
PP2A-B'gamma physically interacts with the cytoplasmic form of aconitase and PP2A-B'gamma impacts reactive oxygen species homeostasis by controlling the abundance of specific alternative oxidase isoforms, AOX1A and AOX1D, in leaf mitochondria.[PP2A-B'gamma] PMID: 25307043
PP2A-B'gamma is a positive regulator of flowering that contributes to the repression of the major flowering repressor gene FLC in Arabidopsis. PMID: 23976921
PP2A-B'gamma modulates the extent of ER-stress through dephosphorylation of CALRETICULIN 1 PMID: 22041935
The specific B'gamma subunit of PP2A is functionally connected to CPR5 and operates in the basal repression of defense responses under low irradiance. [PP2A-B'gamma] PMID: 21571669

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Subcellular Location

Cytoplasm, cytosol. Nucleus.

Protein Families

Phosphatase 2A regulatory subunit B56 family

Tissue Specificity

Expressed ubiquitously at low levels. Expressed in roots, emerging lateral roots, cotyledons, leaves, floral stalks and flowers.

Database Links

KEGG: ath:AT4G15415

STRING: 3702.AT4G15415.1

UniGene: At.4343

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B'GAMMA Antibody

Product Details

Customer Reviews and Q&A

Target Background