Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) PP2AA1 Polyclonal antibody

Uniprot No.

Q38845

Target Names

PP2AA1

Alternative Names

PP2AA1 antibody; EER1 antibody; RCN1 antibody; REGA antibody; At1g25490 antibody; F2J7.19Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform antibody; AtA alpha antibody; PP2A antibody; subunit A antibody; alpha isoform antibody; PR-65 A antibody; Protein ROOTS CURL IN NAPHTHYLPHTHALAMIC ACID 1 antibody; Protein enhancer of ethylene-response 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana PP2AA1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The A subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Seems to act as a positive regulator of PP2A catalytic activity. Confers resistance to phosphatase inhibitors such as okadaic acid and cantharidin. Involved during developmental process such as seedling and floral developments, root gravitropism, and stomatal opening regulation. Involved in the regulation of auxin efflux, especially during basipetal (tips to base) auxin transport in roots, and appears to contribute to the perception of auxin efflux inhibitors such as 1-N-naphthylphthalamic acid (NPA) and to semicarbazone I (substituted phenylsemicarbazone of 2-acetylarylcarboxylic acids) (SCB-I). Modulates the magnitude of ethylene response in the hypocotyl and stem, and functions as a general positive transducer of early ABA signaling. The holoenzyme composed of PP2AA1, PP2A4 and B'ZETA or B'ETA acts as negative regulator of plant innate immunity by controlling BAK1 phosphorylation state and activation in surface-localized immune receptor complexes.

Gene References into Functions

The RCN1 protein affects overall hypocotyl elongation via negative regulation of ethylene synthesis in etiolated seedlings. PMID: 16798939
This study showed that hypocotyl and root growth exhibit different requirements for RCN1-mediated regulation of PP2A activity. PMID: 18162590
RCN1 controls signaling between methyl jasmonate and abscisic acid. MeJA and ABA failed to induce stomatal closure in RCN1 mutants. PMID: 18650210

Subcellular Location

Cytoplasm, cytosol. Nucleus.

Protein Families

Phosphatase 2A regulatory subunit A family

Tissue Specificity

Mostly expressed in cell-dividing tissues such as apical meristems. Ubiquitous, with higher levels in roots and flowers (at protein level).

Database Links

KEGG: ath:AT1G25490

STRING: 3702.AT1G25490.1

UniGene: At.160

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA656432XA01DOA
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

PP2AA1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background