cN-IIIB Antibody

Code CSB-PA151043XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) cN-IIIB Polyclonal antibody
Uniprot No.
Target Names
cN-IIIB
Alternative Names
cN-IIIB antibody; CG33627-methylguanosine phosphate-specific 5'-nucleotidase antibody; 7-methylguanosine nucleotidase antibody; EC 3.1.3.91 antibody; Cytosolic 5'-nucleotidase IIIB antibody; EC 3.1.3.5 antibody; N(7)-methylguanylate 5'-phosphatase antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) cN-IIIB protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate. Also able to mediate hydrolysis of diphosphate (m(7)GDP) to 7-methylguanosine and 2 inorganic phosphate with lower activity. The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates.
Gene References into Functions
  1. Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP as a substrate. PMID: 24603684
  2. analysis of Drosophila and human 7-methyl GMP-specific nucleotidases PMID: 23223233
Protein Families
Pyrimidine 5'-nucleotidase family
Database Links

KEGG: dme:Dmel_CG3362

STRING: 7227.FBpp0072168

UniGene: Dm.769

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