atg-16.2 Antibody

Code CSB-PA255422XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans atg-16.2 Polyclonal antibody
Uniprot No.
Target Names
atg-16.2
Alternative Names
atg-16.2 antibody; K06A1.5Autophagic-related protein 16.2 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans atg-16.2 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Most likely a component of the atg-5-atg-12-atg-16.1/atg-16.2 complex, which is recruited to the preautophagosomal membrane and associates with lgg-2 to promote autophagosome formation. Plays a role in the recruitment of lipidated lgg-1 probably to the autophagosome membrane to promote autophagosome formation. Furthermore, association with atg-5 is required for the nucleation of lgg-1 positive autophagosomes. Although its role in autophagosome formation may be distinct to the role of atg-16.2, it functions in a partially redundant manner with atg-16.1 to regulate autophagic processes. In a daf-18/PTEN- and daf-16/FOXO-dependent manner, required for maintaining the numbers of germ stem cell progenitors in the gonad during the late phases of larval development.
Gene References into Functions
  1. C. elegans ATG-16s are involved in specifying LGG-1 puncta formation; the two ATG-16 homologs have partially redundant yet distinct functions in the aggrephagy pathway. PMID: 24185444
Subcellular Location
Cytoplasm. Cell membrane.
Protein Families
WD repeat tipD family
Tissue Specificity
Expressed in neurons, pharyngeal muscles, body wall muscle cells and intestinal cells.
Database Links

KEGG: cel:CELE_K06A1.5

STRING: 6239.K06A1.5.1

UniGene: Cel.7602

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