ABP140 Antibody

Code CSB-PA801993XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ABP140 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ABP140 antibody; TRM140 antibody; YOR239W antibody; YOR240WtRNA(Thr antibody; cytosine(32)-N(3))-methyltransferase antibody; EC antibody; Actin-binding protein of 140 kDa antibody; tRNA methyltransferase of 140 kDa antibody
Raised in
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ABP140 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr) and tRNA(Ser). Binds F-actin and shows weak F-actin cross-linking activity.
Gene References into Functions
  1. S. cerevisiae Trm140 has two recognition modes for 3-methylcytidine modification of the anticodon loop of tRNA substrates. PMID: 28003514
  2. a model of translational coupling was proposed, in which ABP140 mRNA is tethered to actin cables via its nascent protein product and is transported to the distal pole by actin retrograde flow. [review] PMID: 21792172
  3. A domain of the actin binding protein Abp140 is the yeast methyltransferase responsible for 3-methylcytidine modification in the tRNA anti-codon loop PMID: 21518804
  4. Actin-binding protein ABP140 is a methyltransferase for 3-methylcytidine at position 32 of tRNAs in Saccharomyces cerevisiae PMID: 21518805
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton. Note=Cytoplasmic and cortical cytoskeleton.
Protein Families
Methyltransferase superfamily, METL family
Database Links

KEGG: sce:YOR239W

STRING: 4932.YOR239W

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