Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABCB1 Polyclonal antibody

Uniprot No.

Q9ZR72

Target Names

ABCB1

Alternative Names

ABCB1 antibody; MDR1 antibody; PGP1 antibody; At2g36910 antibody; T1J8.9ABC transporter B family member 1 antibody; ABC transporter ABCB.1 antibody; AtABCB1 antibody; Multidrug resistance protein 1 antibody; P-glycoprotein 1 antibody; AtPgp1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABCB1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Auxin efflux transporter that acts as a negative regulator of light signaling to promote hypocotyl elongation. Mediates the accumulation of chlorophyll and anthocyanin, as well as the expression of genes in response to light. Participates directly in auxin efflux and thus regulates the polar (presumably basipetal) auxin transport (from root tips to root elongating zone). Transports also some auxin metabolites such as oxindoleacetic acid and indoleacetaldehyde. Involved in diverse auxin-mediated responses including gravitropism, phototropism and lateral root formation. Confers resistance to herbicides such as dicamba, pendimethalin, oryzalin, and monosodium acid methanearsonate (MSMA), but not to herbicides such as glyphosate, atrazine, bentazon and fluazifop-p-butyl. Mediates also resistance to xenobiotics such as cycloheximide and the cytokinin N6-(2-isopentenyl)adenine (2IP).

Gene References into Functions

ABCB1 plays a major role during anther development, while ABCB19 has a synergistic role. PMID: 25626615
TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation. PMID: 24313847
These results suggest a direct involvement of AtPGP1 in auxin transport processes controlling plant growth PMID: 24211579
TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm. PMID: 23321285
Evidence was provided that AGC protein kinase phosphorylation has a dual, counter-active impact on ABCB1 activity that is coordinated by TWD1-PID interaction. PMID: 22549467
The functional characterization of two closely related ABC (ATP-binding cassette) transporter genes, AtMDR1 and AtPGP1, in light and auxin responses. PMID: 15908594
The localization, activity, substrate specificity and inhibitor sensitivity of AtPGP1 are reported. PMID: 16212599
Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoprotein PMID: 16887800
Data show that root hair cell-specific overexpression of auxin efflux transporter PGP1 decreased root hair elongation. PMID: 18156217

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Subcellular Location

Cell membrane; Multi-pass membrane protein. Note=Non-polar distribution in apical cells. Predominant basal (top) localization in root tissues above the elongation zone, especially in mature cortical and endodermal cells. Basal and apical localization in the root elongation zone.

Protein Families

ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily

Tissue Specificity

Ubiquitous, with high levels in peduncles. Mostly localized in young developing tissues, including meristems, as well as root and shoot apices.

Database Links

KEGG: ath:AT2G36910

STRING: 3702.AT2G36910.1

UniGene: At.142

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ABCB1 Antibody

Product Details

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Target Background