ABCB1 Antibody

Code CSB-PA230252XA01DOA
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Product Details

Full Product Name Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABCB1 Polyclonal antibody
Uniprot No. Q9ZR72
Target Names ABCB1
Alternative Names ABCB1 antibody; MDR1 antibody; PGP1 antibody; At2g36910 antibody; T1J8.9ABC transporter B family member 1 antibody; ABC transporter ABCB.1 antibody; AtABCB1 antibody; Multidrug resistance protein 1 antibody; P-glycoprotein 1 antibody; AtPgp1 antibody
Raised in Rabbit
Species Reactivity Arabidopsis thaliana
Immunogen Recombinant Arabidopsis thaliana ABCB1 protein
Immunogen Species Arabidopsis thaliana (Mouse-ear cress)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

(From Uniprot)
Auxin efflux transporter that acts as a negative regulator of light signaling to promote hypocotyl elongation. Mediates the accumulation of chlorophyll and anthocyanin, as well as the expression of genes in response to light. Participates directly in auxin efflux and thus regulates the polar (presumably basipetal) auxin transport (from root tips to root elongating zone). Transports also some auxin metabolites such as oxindoleacetic acid and indoleacetaldehyde. Involved in diverse auxin-mediated responses including gravitropism, phototropism and lateral root formation. Confers resistance to herbicides such as dicamba, pendimethalin, oryzalin, and monosodium acid methanearsonate (MSMA), but not to herbicides such as glyphosate, atrazine, bentazon and fluazifop-p-butyl. Mediates also resistance to xenobiotics such as cycloheximide and the cytokinin N6-(2-isopentenyl)adenine (2IP).
Gene References into Functions
  1. ABCB1 plays a major role during anther development, while ABCB19 has a synergistic role. PMID: 25626615
  2. TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation. PMID: 24313847
  3. These results suggest a direct involvement of AtPGP1 in auxin transport processes controlling plant growth PMID: 24211579
  4. TWD1 promotes lateral ABCB-mediated auxin efflux via protein-protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm. PMID: 23321285
  5. Evidence was provided that AGC protein kinase phosphorylation has a dual, counter-active impact on ABCB1 activity that is coordinated by TWD1-PID interaction. PMID: 22549467
  6. The functional characterization of two closely related ABC (ATP-binding cassette) transporter genes, AtMDR1 and AtPGP1, in light and auxin responses. PMID: 15908594
  7. The localization, activity, substrate specificity and inhibitor sensitivity of AtPGP1 are reported. PMID: 16212599
  8. Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoprotein PMID: 16887800
  9. Data show that root hair cell-specific overexpression of auxin efflux transporter PGP1 decreased root hair elongation. PMID: 18156217

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Subcellular Location Cell membrane; Multi-pass membrane protein. Note=Non-polar distribution in apical cells. Predominant basal (top) localization in root tissues above the elongation zone, especially in mature cortical and endodermal cells. Basal and apical localization in the root elongation zone.
Protein Families ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily
Tissue Specificity Ubiquitous, with high levels in peduncles. Mostly localized in young developing tissues, including meristems, as well as root and shoot apices.
Database Links

KEGG: ath:AT2G36910

STRING: 3702.AT2G36910.1

UniGene: At.142

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