ABCB1 is a transmembrane protein predominantly found in cells lining the blood-brain barrier, liver, intestine, and kidneys. By leveraging the energy from ATP breakdown, this protein transports drugs and other substances out of cells against their concentration gradients, which in turn reduces the intracellular drug levels and contributing to multidrug resistance. ABCB1 plays a pivotal role in cellular detoxification and homeostasis, and significantly impacts drug absorption, distribution, metabolism, and excretion, making it a central player in drug resistance. Aberrant ABCB1 expression is linked to treatment resistance across a range of diseases, including the multidrug resistance observed in tumors.
Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial (CSB-EP001046HU2)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
ABCB1 Recombinant Monoclonal Antibody (CSB-RA983422A0HU)
Validated Data
IHC image of CSB-RA983422A0HU diluted at 1:100 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence staining of HepG2 cell with CSB-RA983422A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 583-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The following ABCB1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ABCB1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA11737D0Rb | ABCB1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA11737B0Rb | ABCB1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA11737C0Rb | ABCB1 Antibody, FITC conjugated | Human | |
CSB-PA11737A0Rb | ABCB1 Antibody | Human | ELISA, IHC |
CSB-PA030050 | ABCB1 Antibody | Human,Mouse,Rat | IHC, ELISA |
CSB-PA598314 | ABCB1 Antibody | Human | ELISA,IHC |
CSB-RA983422A0HU | ABCB1 Recombinant Monoclonal Antibody | Human | ELISA, IHC, IF |
ABCB1 Antibodies for Arabidopsis thaliana (Mouse-ear cress)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA230252XA01DOA | ABCB1 Antibody | Arabidopsis thaliana | ELISA, WB (ensure identification of antigen) |
ABCB1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-EP001046HU1 | Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial | E.coli |
CSB-YP001046HU CSB-EP001046HU CSB-BP001046HU CSB-MP001046HU CSB-EP001046HU-B |
Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CSB-EP001046HU2 | Recombinant Human ATP-dependent translocase ABCB1 (ABCB1), partial | E.coli |
ABCB1 Proteins for Dictyostelium discoideum (Slime mold)
Code | Product Name | Source |
---|---|---|
CSB-YP684501DKK CSB-EP684501DKK CSB-BP684501DKK CSB-MP684501DKK CSB-EP684501DKK-B |
Recombinant Dictyostelium discoideum ABC transporter B family member 1 (abcB1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ABCB1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-YP001046RA CSB-EP001046RA CSB-BP001046RA CSB-MP001046RA CSB-EP001046RA-B |
Recombinant Rat Multidrug resistance protein 1 (Abcb1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ABCB1 Proteins for Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Code | Product Name | Source |
---|---|---|
CSB-YP001046DXU CSB-EP001046DXU CSB-BP001046DXU CSB-MP001046DXU CSB-EP001046DXU-B |
Recombinant Cricetulus griseus Multidrug resistance protein 1 (ABCB1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
ABCB1 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E11709h | Human permeability glycoprotein,P-gp/ABCB1 ELISA Kit | serum, plasma, ascitic fluid, tissue homogenates | 0.78 ng/mL |