Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABCB4 Polyclonal antibody

Uniprot No.

O80725

Target Names

ABCB4

Alternative Names

ABCB4 antibody; MDR4 antibody; PGP4 antibody; At2g47000 antibody; F14M4.17ABC transporter B family member 4 antibody; ABC transporter ABCB.4 antibody; AtABCB4 antibody; Multidrug resistance protein 4 antibody; P-glycoprotein 4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ABCB4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Auxin influx transporter that mediates the transport of auxin in roots. Contributes to the basipetal transport in hypocotyls and root tips by establishing an auxin uptake sink in the root cap. Confers sensitivity to 1-N-naphthylphthalamic acid (NPA). Regulates the root elongation, the initiation of lateral roots and the development of root hairs. Can transport IAA, indole-3-propionic acid, NPA syringic acid, vanillic acid and some auxin metabolites, but not 2,4-D and 1-naphthaleneacetic acid.

Gene References into Functions

2,4-dichlorophenoxyacetic acid (2,4-D) is a non-competitive inhibitor of indole-3-acetic acid (IAA) transport by ABCB4 and indicate that ABCB4 is a target of 2,4-D herbicidal activity. PMID: 21992190
ABCB4 localizes in a nonpolar manner and is stably retained in the plasma membrane. PMID: 22492845
Mutations in Multidrug Resistance-Like1 (MDR1) reduced acropetal auxin transport in roots by 80% without affecting basipetal transport. Conversely, mutations in MDR4 blocked 50% of basipetal transport without affecting acropetal transport. PMID: 17557805
Results suggest that PGP4 is an auxin efflux transporter in plants and that its trafficking to the plasma membrane involves both BFA-sensitive and -insensitive pathways. PMID: 18156217

Subcellular Location

Cell membrane; Multi-pass membrane protein. Note=Non-polar distribution in apical cells. Apical (bottom) localization in mature root cells. Basal (top) localization in the root elongation zone.

Protein Families

ABC transporter superfamily, ABCB family, Multidrug resistance exporter (TC 3.A.1.201) subfamily

Tissue Specificity

Mostly expressed in roots, especially in the root elongation zone and lateral roots. In mature portion of the root, expressed in the epidermis and cortex. In the root elongation zone, confined to epidermis. In root tips, present in the root cap, S3 colume

Database Links

KEGG: ath:AT2G47000

STRING: 3702.AT2G47000.1

UniGene: At.43921

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ABCB4 Antibody

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