ABI3 Antibody

Code CSB-PA274875XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABI3 Polyclonal antibody
Uniprot No.
Target Names
ABI3
Alternative Names
ABI3 antibody; At3g24650 antibody; MSD24.2B3 domain-containing transcription factor ABI3 antibody; Protein ABSCISIC ACID-INSENSITIVE 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ABI3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Participates in abscisic acid-regulated gene expression during seed development. Regulates the transcription of SGR1 and SGR2 that are involved in leaf and embryo degreening.
Gene References into Functions
  1. ABI3 mediates dehydration stress signaling in Arabidopsis through regulation of a group of genes that play a role primarily during stress recovery phase. PMID: 27457990
  2. The abi3-1 dog1-1 double mutant produced green seeds which are highly abscisic acid (ABA) insensitive, phenocopying severe abi3 mutants, indicating that dog1-1 acts as an enhancer of the weak abi3-1 allele and thus revealing a genetic interaction between both genes. PMID: 26729600
  3. TIP3;1 and TIP3;2 promoters could be activated by ABI3 in the presence of abscisic acid (ABA) in Arabidopsis protoplasts. TIP3 proteins were detected in protoplasts transiently expressing ABI3 and in ABI3-overexpressing seedlings treated with ABA. PMID: 26019256
  4. AIP1 interacts with ABAP1, with a plant histone modification "reader" (LHP1) and with non modified histones. Also, expression of ABAP1 and LHP1 target genes were repressed in flower buds of plants with reduced levels of AIP1. PMID: 26538092
  5. This study examined the role of ABSCISIC ACID INSENSITIVE3 (ABI3), FUSCA3 (FUS3) and LEAFY COTYLEDON2 (LEC2), in the production of seed reserves in Arabidopsis. PMID: 25840088
  6. The BES1-TPL-HDA19 repressor complex controls epigenetic silencing of ABI3 and thereby suppresses the abscisic acid signalling output during early seedling development. PMID: 24938150
  7. WRKY41 is an important regulator of ABI3 expression, and hence of seed dormancy. PMID: 24946881
  8. ABI3 controls embryo degreening through Mendel's I locus. PMID: 24043799
  9. Auxin controls seed dormancy through stimulation of abscisic acid signaling by inducing ARF-mediated ABI3 activation in Arabidopsis. PMID: 23986496
  10. Identification of a set of 98 genes forming a basic ABI3 regulon. PMID: 22730287
  11. N-acylethanolamines may act to inhibit early seedling establishment by both ABI3-dependent and ABI3-independent pathways. PMID: 21633189
  12. We suggest that LEC1 controls the expression of the SSP genes in a hierarchical manner, which involves ABI3 and FUS3. PMID: 15695450
  13. The expression of ABI3- and/or ABA-responsive genes and cis-elements in the promoters are discussed PMID: 16463099
  14. ABI3 expression ceases following the completion of germination in both tomato and Arabidopsis seeds, suggesting that expression of this gene does not regulate germination. PMID: 16531465
  15. ABSCISIC ACID-INSENSITIVE3 (ABI3) regulates the developmental expression of HsfA9. PMID: 17220197

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Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly found in the nucleus.
Tissue Specificity
Isoform 2 accumulates only at the end of seed maturation.
Database Links

KEGG: ath:AT3G24650

STRING: 3702.AT3G24650.1

UniGene: At.19959

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