Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ERABP1 Polyclonal antibody

Uniprot No.

P33487

Target Names

ERABP1

Alternative Names

ERABP1 antibody; At4g02980 antibody; T4I9.14Auxin-binding protein 1 antibody; ABP1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ERABP1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Auxin receptor that controls cell elongation and cell division. Involved in embryonic morphogenesis. Acts on the cell cycle, endocycle, cell plate formation, and cell expansion and contributes to the control of auxin-related gene expression. Controls root meristem size and mediates auxin responsiveness. Involved in activation of ROP GTPases in response to auxin and regulation of clathrin-mediated endocytosis in roots. Acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Upon auxin binding, restricts the internalization of PIN proteins by inhibiting clathrin-mediated endocytosis. Involved in the regulation of polar auxin transport. Behaves as a negative regulator of the SCF(TIR1/AFB) signaling pathway, protecting AUX/IAA repressors from degradation. Regulates the expression of cell wall remodeling genes via an SCF(TIR1/AFB)-dependent pathway. Involved in the modulation of hemicellulose xyloglucan structure. Required for rapid auxin-mediated re-orientation of microtubules to regulate cell elongation in roots and dark-grown hypocotyls as well as asymmetric growth during gravitropic responses. Involved in the shade avoidance response. Forms with TMK1 a cell surface auxin perception complex that activates ROP signaling pathways. ABP1 sensing of auxin is important for the ABP1-TMK1 complex formation. Interacts functionally with phytochrome to regulate growth.

Gene References into Functions

ABP1 seems to be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the control of rapid auxin-induced growth. PMID: 28860155
a phyB null allele in the abp1-5 background is likely causative for the long hypocotyl phenotype previously attributed to disrupted ABP1 function. PMID: 26106149
Phenotypes linked to auxin transport were compromised in three complemented abp1 mutants. Red light effects were all compromised in the complemented lines. Auxin-induced expression was delayed after 10 min, and light-induced expression within 60 min. PMID: 25392478
ABP1 affects the expression of a broad range of cell wall-related genes, especially cell wall remodeling genes. PMID: 24424095
Differential auxin regulation of clathrin light chains and clathrin heavy chains membrane association is dependent on ABP1 signaling. PMID: 23424247
The heterozygous abp1/ABP1 insertion mutant has defects in auxin physiology-related responses: higher root slanting angles, longer hypocotyls, agravitropic roots and hypocotyls, aphototropic hypocotyls, and decreased apical dominance. PMID: 21223392
Study demonstrates that ABP1 mediates a nontranscriptional auxin signaling that regulates process of clathrin-mediated endocytosis and suggests that this signaling may be essential for the developmentally important feedback of auxin on its own transport. PMID: 20887896
AtRma2 is likely involved in the cellular regulation of ABP1 expression levels. PMID: 20152813
ABP1 is a key regulator for root growth and is required for auxin-mediated responses PMID: 19777056
ABP1 acts as a coordinator of cell division and expansion, with local auxin levels influencing ABP1 effectiveness. PMID: 18952781

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Subcellular Location

Endoplasmic reticulum lumen. Cell membrane; Peripheral membrane protein.

Database Links

KEGG: ath:AT4G02980

STRING: 3702.AT4G02980.1

UniGene: At.148

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ERABP1 Antibody

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