ab Antibody

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Code	CSB-PA634502XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) ab Polyclonal antibody

Uniprot No.

Q24174

Target Names

Alternative Names

ab antibody; clu antibody; CG43860 antibody; Protein abrupt antibody; Protein clueless antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) ab protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Expression is vital for development; may be involved in transcriptional regulation. In embryos, muscle specific expression is required for segmental nerve b (SNb) motoneuron target recognition within ventral longitudinal muscles. Has a role in establishing and maintaining embryonic muscle attachments, adult sensory cell formation (macrochaetae) and morphogenesis of adult appendages (legs, antenna aristae and male external genitalia). Has a role in the morphogenesis of the class I dendritic neurons: selective expression of ab in class I da neurons plays a pivotal role in forming dendritic arbors, which are characteristic of the class I cells. The development of more complex arbors of class II-IV neurons depends on the absence of ab.

Gene References into Functions

ab is essential for proper wing vein patterning both in Tribolium and Drosophila. PMID: 26687509
The transcription factors Abrupt (Ab) and Knot (Kn) act as selectors of distinct dendritic arbor morphologies in two classes of Drosophila sensory neurons, termed class I and class IV, respectively. PMID: 24290980
Ab endows characteristics of dendritic morphogenesis of the class I ofdendritic arborization (da)neurons. PMID: 15363392
The dendritic branching can be suppressed by neuronal subtype-specific transcription factors in a cell-autonomous and dosage-dependent manner. PMID: 15363393
Abrupt is a repressor of ecdysone signalling and border-cell migration in ovarian follicles. Abrupt represents a node of integration for steroid hormone and JAK-STAT signals. PMID: 19350016

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Subcellular Location

Nucleus.

Tissue Specificity

Expressed in CNS midline cells during embryonic stages 9-13. Expression also seen in cells of the stomagastric nervous system. Segmentally repeated stripes of ectodermal expression appear at stage 11 that become uniform by stage 12 and throughout embryoge

Database Links

KEGG: dme:Dmel_CG43860

UniGene: Dm.1729

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