ACD11 Antibody

Code CSB-PA127374XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ACD11 Polyclonal antibody
Uniprot No.
Target Names
ACD11
Alternative Names
ACD11 antibody; At2g34690 antibody; T29F13.10Accelerated cell death 11 antibody; Ceramide-1-phosphate transfer protein ACD11 antibody; Glycolipid transfer protein domain-containing protein ACD11 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ACD11 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Exhibits selective intermembrane transfer of ceramide-1-phosphate (C1P) and phytoceramide-1-phosphate. Does not transport ceramide (Cer) or GalCer, suggesting a requirement for phosphate in the headgroup for functionality. Transports in vitro sphingosine, but not glycosphingolipids. Has also some in vitro activity with sphingomyelin, a lipid not detected in plant tissues. The transport function may be not directly involved in regulating cell death. Rather, perturbations in the function of ACD11 or related components could be monitored by R-proteins, which then mediate defense and programmed cell death (PCD), as proposed in the guard hypothesis. C1P transfer is stimulated by phosphatidylserine in C1P source vesicles. Regulates autophagy, inflammasome mediated IL1B and IL18 processing, and pyroptosis, but not apoptosis.
Gene References into Functions
  1. In acd11-1 null mutant, normally low ceramide-1-phosphate increases, but phytoceramide rises acutely. PMID: 24412362
  2. Autoimmunity in Arabidopsis acd11 is mediated by epigenetic regulation of an immune receptor. PMID: 20949080
  3. Lazarus1 (AT4G38360)contributes to programmed cell death associated with acd11 and the hypersensitive response. PMID: 20830211
  4. generated site-specific mutants in ACD11 based on homologous GLTP residues required for glycolipid transfer. We show that these ACD11 mutant forms are impaired in their in vitro transfer activity of sphingolipids PMID: 18657186
  5. ACD11 function could be related to the regulation of membrane compartments. PMID: 18845362

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Subcellular Location
Cytoplasm.
Protein Families
GLTP family
Database Links

KEGG: ath:AT2G34690

STRING: 3702.AT2G34690.1

UniGene: At.12512

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