yghJ Antibody

Code CSB-PA317243XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) yghJ Polyclonal antibody
Uniprot No.
Target Names
yghJ
Alternative Names
yghJ antibody; b4466 antibody; JW5925 antibody; ECK2968Putative lipoprotein AcfD homolog antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) yghJ protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane.
Gene References into Functions
  1. Differential regulation of the surface-exposed and secreted SslE lipoprotein in extraintestinal pathogenic Escherichia coli has been reported. PMID: 27598999
  2. The authors showed that YghJ can cause extensive tissue damage in mouse ileum and is also able to induce significant fluid accumulation in a dose dependent manner in a mouse ileal loop (MIL) assay. PMID: 28212863
  3. SslE expression not only increases bacterial growth in the presence of mucosal substrates but it also facilitates E. coli penetration of the mucus. PMID: 25789808
  4. Importance of SslE in E. coli colonization of mucosal surfaces, which reinforce the use of this antigen as a component of a broadly protective vaccine against pathogenic E. coli species. PMID: 24809621
  5. YghJ is a highly conserved metalloprotease that influences intestinal colonization of enterotoxigenic Escherichia coli by degrading the major mucins in the small intestine, MUC2 and MUC3. PMID: 24478067

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Subcellular Location
Cell inner membrane; Lipid-anchor.
Database Links

KEGG: ecj:JW5925

STRING: 316407.85675781

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