Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ACIN1 Polyclonal antibody

Uniprot No.

Q9UKV3

Target Names

ACIN1

Alternative Names

ACIN 1 antibody; ACIN1 antibody; ACINU_HUMAN antibody; Acinus antibody; ACN antibody; Apoptotic chromatin condensation inducer 1 antibody; Apoptotic chromatin condensation inducer in the nucleus antibody; DKFZp667N107 antibody; fSAP152 antibody; functional spliceosome-associated protein 152 antibody; KIAA0670 antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens (Human)

Immunogen

Recombinant Homo sapiens (Human) ACIN1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Component of the ASAP complexes which bind RNA in a sequence-independent manner and are proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets; ACIN1 confers RNA-binding to the complex. The ASAP complex can inhibit RNA processing during in vitro splicing reactions. The ASAP complex promotes apoptosis and is disassembled after induction of apoptosis. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the activity is different from the established EJC assembly and function. Induces apoptotic chromatin condensation after activation by CASP3. Regulates cyclin A1, but not cyclin A2, expression in leukemia cells.

Gene References into Functions

ACIN1 mRNA was highly expressed in platelets of lung cancer patients PMID: 30201066
We conclude that RNPS1 is a key factor for the quality control of mRNAs that is essential for the phenotypes including cell division. PMID: 29366779
Genome-wide identification of RNA targets of Acinus revealed its role in splicing regulation as well as its involvement in other cellular pathways, including cell cycle progression. PMID: 27365209
acinus L was identified as a potential novel mTORC1 target. PMID: 25907765
Differences in the nuclear localization of Acinus-S' and Acinus-L may suggest that these two isoforms have different functional roles. PMID: 25079509
the ligand-dependent splicing activity of Acinus is related to the retinoic acid-activated retinoic acid receptor PMID: 25205379
An electronegative region on acinusS restricts SRPK2 phosphorylation to a single specific site. PMID: 24444330
Chronic low-dose radiation increases the levels of AKT and acinus proteins via NF-kappaB activation PMID: 23205493
Akt phosphorylation of acinus on serine 422 and 573 results in its resistance to caspase cleavage in the nucleus and the inhibition of acinus-dependent chromatin condensation. PMID: 16177823
Acinus is not involved in DNA condensation but rather has a role in internucleosomal DNA cleavage during programmed cell death PMID: 16537548
We found hypermethylation of the ACIN1 gene in early stage lung adenocarcinoma. The role of methylation status in the development and malignant transformation of lung adenocarcinoma requires clarification. PMID: 17409846
identify Acinus-S' as a novel RAR-interacting protein[Acinus-S'] PMID: 18250153
The authors report here that AAC-11, a survival protein whose expression prevents apoptosis that occurs on deprivation of growth factors, physiologically binds to Acinus and prevents Acinus-mediated DNA fragmentation. PMID: 19387494
A critical role for AKT activation in protecting cells from ionizing radiation-induced apoptosis and the regulation of acinus gene expression is reported. PMID: 19615784
Study shows that transcription corepressor CtBP2 directly binds acinus, which is regulated by nerve growth factor (NGF), inhibiting its stimulatory effect on cyclin A1, but not cyclin A2, expression in leukemia. PMID: 19668232

Hide All

Subcellular Location

Nucleus. Nucleus speckle. Nucleus, nucleoplasm. Note=Phosphorylation on Ser-1180 by SRPK2 redistributes it from the nuclear speckles to the nucleoplasm.

Tissue Specificity

Ubiquitous. The Ser-1180 phosphorylated form (by SRPK2) is highly expressed and phosphorylated in patients with myeloid hematologic malignancies.

Database Links

HGNC: 17066

OMIM: 604562

KEGG: hsa:22985

STRING: 9606.ENSP00000262710

UniGene: Hs.124490

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ACIN1 Antibody

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