ACT1 Antibody

Code CSB-PA001205XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ACT1 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
ACT1 antibody; ABY1 antibody; END7 antibody; YFL039CActin antibody
Raised in
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ACT1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Purification Method
Protein A/G
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
ELISA, WB (ensure identification of antigen)
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Gene References into Functions
  1. Act1 protein affects tombusvirus recombination in yeast . PMID: 26773384
  2. Using multi-color total internal reflection fluorescence microscopy, the study shows that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament sides. PMID: 26299936
  3. Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2alpha phosphorylation. PMID: 24936063
  4. Heat shock-induced processing of actin assembly protein Lsb1 is involved in prion inheritance. PMID: 25143386
  5. Data suggest that a glycolytic multi-enzyme complex assembles in cytoplasm of S. cerevisiae in association with F-actin (filamentous actin) but not in association with G-actin (monomeric globular actin). PMID: 23763840
  6. Lsb1 and/or Lsb2 full-length proteins inhibit Las17-mediated actin polymerization PMID: 23577202
  7. data suggest that the R256H actin mutation alters filament conformation resulting in filament instability and misregulation by formin PMID: 22753406
  8. analysis of the molecular mechanism of plasma membrane-actin cytoskeleton coupling mediated by cooperative action of epsin Ent1 and the HIP1R homolog Sla2 in yeast Saccharomyces cerevisiae PMID: 22927393
  9. Data suggest that the region surrounding residue 204 is involved in interactions that change depending on the phosphorylation state of the bound nucleotide that might reflect different conformations of F-actin subunits. PMID: 19935871
  10. Vid24p and Sec28p are present at actin patches during glucose starvation. PMID: 19892709
  11. Two recessive mutations, act1-301 in the actin gene and sla2-82 in a gene involved in cortical actin patch assembly, were identified. PMID: 16547104
  12. An unanticipated role for Aip1 and cofilin in promoting rapid turnover of yeast actin cables was revealed. PMID: 16611742
  13. The stabilization of the actin cytoskeleton caused by deletion of Sla1p or End3p leads to hyperactivation of the Ras signaling pathway. PMID: 16914733
  14. Study found 208 genes that have deleterious complex haploinsufficient (CHI) interactions with actin, including several actin-binding protein genes, and nearly half of the CHI genes have defects in actin organization when deleted. PMID: 17167106
  15. In a single ATP-driven cycle, PLP2-CCT-ACT1 complexes yield 30-fold more native actin than CCT-ACT1 complexes. PMID: 19501098

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin family
Database Links

KEGG: sce:YFL039C

STRING: 4932.YFL039C

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