Act5C Antibody

Code CSB-PA319240XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Act5C Polyclonal antibody
Uniprot No.
Target Names
Act5C
Alternative Names
Act5C antibody; CG4027Actin-5C antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Act5C protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.; Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction.
Gene References into Functions
  1. These findings revise the existing spermiation model in Drosophila and suggest that somatic cells can actively oppose mechanical cell invasion attempts using calibrated F-actin dynamics in situ PMID: 27546008
  2. Data suggest that the molecular and structural mechanism of actin polymerization by Spire should apply to other actin-binding proteins that contain WH2 domains in tandem. PMID: 22106272
  3. actin filament subunits were directly modified by Mical on their conserved pointed-end, critical for filament assembly; Mical posttranslationally oxidized the methionine 44 residue within D-loop of actin, severing filaments and decreasing polymerization PMID: 22116028
  4. essential cytoplasmic actin isoform PMID: 12034866
  5. A mutational strategy is used to express monomeric 5C actin that is incapable of polymerization, able to bind skeletal myosin subfragment 1 and form a homogeneous complex, an ideal candidate for structural studies of actin complexes with motor proteins. PMID: 15350141

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Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin family
Database Links

KEGG: dme:Dmel_CG4027

STRING: 7227.FBpp0100124

UniGene: Dm.2951

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