addB Antibody

Code CSB-PA328410XA01BRJ
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Product Details

Full Product Name
Rabbit anti-Bacillus subtilis (strain 168) addB Polyclonal antibody
Uniprot No.
Target Names
addB
Alternative Names
addB antibody; BSU10620 antibody; ATP-dependent helicase/deoxyribonuclease subunit B antibody; EC 3.1.-.- antibody; EC 3.6.4.12 antibody; ATP-dependent helicase/nuclease AddB antibody
Raised in
Rabbit
Species Reactivity
Bacillus subtilis (strain 168)
Immunogen
Recombinant Bacillus subtilis (strain 168) addB protein
Immunogen Species
Bacillus subtilis (strain 168)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.
Gene References into Functions
  1. Chi hotspots trigger a conformational change in the helicase-like domain of AddAB to activate homologous recombination. PMID: 26762979
  2. transient binding of AddAB to chi(Bs) is an integral part of the AddAB-chi(Bs) interaction and propose that this molecular event underlies a general mechanism for regulating the biochemical activities and biological functions of RecBCD-like enzymes PMID: 16632468
  3. Results show that the AddAB enzyme complex contains two active nuclease domains, each of which is dedicated to cleavage of one specific DNA strand. PMID: 17570399
Protein Families
Helicase family, AddB/RexB type 1 subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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