ADF1 Antibody

Code CSB-PA657196XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADF1 Polyclonal antibody
Uniprot No.
Target Names
ADF1
Alternative Names
ADF1 antibody; At3g46010 antibody; F16L2_220Actin-depolymerizing factor 1 antibody; ADF-1 antibody; AtADF1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ADF1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Actin-depolymerizing protein. Stimulates F-actin depolymerization. Involved in plant development, cell organ expansion and flowering by controlling breakdown of thick actin cables. Severs actin filaments or bundles and promotes actin cytoskeleton disassembly. Binds monomeric actin (G-actin) with a marked preference for the ADP-loaded form and inhibits the rate of nucleotide exchange on G-actin.
Gene References into Functions
  1. Data show that 14-3-3 lambda is required for actin depolymerizing factor 1 (ADF1) phosphorylation and subcellular localization. PMID: 26345162
  2. These results demonstrate a regulatory function of Arabidopsis CDPK6 in the N-terminal phosphorylation of AtADF1. PMID: 23903947
  3. Arabidopsis AtADF1 is functionally affected by mutations on actin binding sites. PMID: 23190411
Subcellular Location
Cytoplasm, cytoskeleton.
Protein Families
Actin-binding proteins ADF family
Tissue Specificity
Expressed in vascular tissues of all organs.
Database Links

KEGG: ath:AT3G46010

STRING: 3702.AT3G46010.2

UniGene: At.21187

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