ADF7 Antibody

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Code	CSB-PA731551XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADF7 Polyclonal antibody

Uniprot No.

Q67ZM4

Target Names

ADF7

Alternative Names

ADF7 antibody; At4g25590 antibody; M7J2.40Actin-depolymerizing factor 7 antibody; ADF-7 antibody; AtADF7 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ADF7 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Actin-depolymerizing protein. Severs actin filaments (F-actin) and binds to actin monomers. Binds monomeric actin (G-actin) with a marked preference for the ADP-loaded form and inhibits the rate of nucleotide exchange on G-actin. Required for pollen tube growth. Promotes turnover of longitudinal actin cables by severing actin filaments in pollen tubes.

Gene References into Functions

ADF7 evolved to promote turnover of longitudinal actin cables by severing actin filaments in pollen tubes. PMID: 24058157
ADF7 and ADF10 play distinct roles during pollen development and pollen tube growth. PMID: 22751315
ADF7 and ADF10 were differentially expressed with the ADF7 signal appearing in the microspore stage and that of ADF10 only during the polarized microspore stage. ADF7 was associated with the subapical actin fringe in germinating pollen grains. PMID: 21632657

Subcellular Location

Cytoplasm, cytoskeleton.

Protein Families

Actin-binding proteins ADF family

Tissue Specificity

Specifically expressed in pollen.

Database Links

KEGG: ath:AT4G25590

STRING: 3702.AT4G25590.1

UniGene: At.32246

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ADF7 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background