ADH1 Antibody

Code CSB-PA174355XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ADH1 Polyclonal antibody
Uniprot No.
Target Names
ADH1
Alternative Names
ADH1 antibody; ADC1 antibody; YOL086C antibody; O0947Alcohol dehydrogenase 1 antibody; EC 1.1.1.1 antibody; Alcohol dehydrogenase I antibody; YADH-1 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ADH1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
This isozyme preferentially catalyzes the conversion of primary unbranched alcohols to their corresponding aldehydes. Also shows activity toward secondary alcohols.
Gene References into Functions
  1. Subunit B may represent an intermediate in the mechanism after coenzyme and alcohol bind and before the conformation changes to the closed form and the alcohol oxygen binds to the zinc and displaces Glu-67. PMID: 26743849
  2. key role of Y295C mutation in HMF reduction by Adh1p. PMID: 25287956
  3. Glucose 6-phosphate and alcohol dehydrogenase activities are components of dynamic macromolecular depot structures. PMID: 25662817
  4. analysis of the oxidation of yeast alcohol dehydrogenase-1 by hydrogen peroxide in vitro PMID: 17203966
  5. A yeast strain with an extra copy of the ADH1 gene (2xADH1) demonstrated increased survival in stationary phase and a 30% extension in replicative life span. PMID: 17975074
  6. The identification and characterization of a mutated alcohol dehydrogenase 1 from the industrial Saccharomyces cerevisiae strain TMB3000 that mediates the NADH-dependent reduction of 5-hydroxymethylfurfural (HMF) to 2,5-bis-hydroxymethylfuran. PMID: 18302314
  7. a sequence -YSGVCHTDLHAWHGDWPLPVK- in the polypeptide chain possesses chaperone-like activity PMID: 18515108
  8. mutation of the ADH1 gene improves lactate production in the yeast Saccharomyces cerevisiae PMID: 19122995

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Subcellular Location
Cytoplasm.
Protein Families
Zinc-containing alcohol dehydrogenase family
Database Links

KEGG: sce:YOL086C

STRING: 4932.YOL086C

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