afp18 Antibody

Code CSB-PA270774XA01GIA
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Product Details

Full Product Name
Rabbit anti-Yersinia ruckeri serotype O1 (strain ATCC 29473 / DSM 18506 / JCM 15110 / NCIMB 2194 / NCTC 12986 / 2396-61) afp18 Polyclonal antibody
Uniprot No.
Target Names
afp18
Alternative Names
afp18 antibody; DJ39_2720 antibody; yruck0001_1570Toxin Afp18 antibody; Antifeeding prophage 18 antibody; Antifeeding protein 18) [Includes: Protein N-acetylglucosaminyltransferase antibody; Protein O-GlcNAc transferase antibody; EC 2.4.1.- antibody; Tyrosine glycosyltransferase)] antibody
Raised in
Rabbit
Species Reactivity
Yersinia ruckeri serotype O1 (strain ATCC 29473 / DSM 18506 / JCM 15110 / NCIMB 2194 / NCTC 12986 / 2396-61)
Immunogen
Recombinant Yersinia ruckeri serotype O1 (strain ATCC 29473 / DSM 18506 / JCM 15110 / NCIMB 2194 / NCTC 12986 / 2396-61) afp18 protein
Immunogen Species
Yersinia ruckeri serotype O1 (strain ATCC 29473 / DSM 18506 / JCM 15110 / NCIMB 2194 / NCTC 12986 / 2396-61)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Toxin component of the prophage tail-derived protein translocation system Afp, which is the causative agent of enteric redmouth disease in salmonid fish species. Mono-O-GlcNAcylates the small GTPase RhoA in eukaryotic host cells at Tyr-34, using UDP-N-acetylglucosamine (UDP-GlcNAc) as the sugar donor. Glycosylation of RhoA results in impaired effector and regulator interaction and inactivation of downstream RhoA signaling which leads to actin filament depolymerization and blocks cytokinesis and gastrulation during zebrafish embryo development. To a lesser extent, is also able to glycosylate other Rho family GTPases (RhoB, RhoC, Rac1, Rac2, Rac3, and Cdc42) in vitro at a switch I tyrosine residue, but not Ras proteins.
Subcellular Location
Secreted. Host cell membrane.
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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