AG Antibody

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Code	CSB-PA325945XA01DOA
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Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AG Polyclonal antibody

Uniprot No.

P17839

Target Names

Alternative Names

AG antibody; At4g18960 antibody; F13C5.130Floral homeotic protein AGAMOUS antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AG protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Probable transcription factor involved in the control of organ identity during the early development of flowers. Is required for normal development of stamens and carpels in the wild-type flower. Plays a role in maintaining the determinacy of the floral meristem. Acts as C class cadastral protein by repressing the A class floral homeotic genes like APETALA1. Forms a heterodimer via the K-box domain with either SEPALATTA1/AGL2, SEPALATTA2/AGL4, SEPALLATA3/AGL9 or AGL6 that could be involved in genes regulation during floral meristem development. Controls AHL21/GIK, a multifunctional chromatin modifier in reproductive organ patterning and differentiation. Induces microsporogenesis through the activation of SPL/NZZ.

Gene References into Functions

findings suggest that AGAMOUS functions to maintain the irreversible state of reproductive development through the negative regulation of floral meristem identity genes and genes involved in organ initiation PMID: 29218596
AP2 does not repress the transcription of AG in the inner two whorls, but instead counteracts AG activity. PMID: 27604611
ARF3 integrates the functions of AGAMOUS and APETALA2 in floral meristem determinacy. PMID: 25187180
The ULT1 and LFY pathways act separately in regulating identity and determinacy at the floral meristem. In particular, they independently induce AG expression in the centre of the flower to terminate meristem activity. PMID: 25288633
FLK and PEP physically interact with proteins involved in AG pre-mRNA processing to secure correct AG function in the floral meristem and flower PMID: 25658099
AGAMOUS directly represses WUS expression by binding to the WUS locus and recruiting, directly or indirectly, PcG that methylates histone H3 Lys-27 at WUS. PMID: 22028461
A model in which the decision whether stamens or petals develop is based on the balance between AP2 and AG activities, rather than the two being mutually exclusive. PMID: 20876650
floral phenotype of bellringer mutants is caused by derepression of AG, suggesting that BLR functions as a transcription repressor PMID: 15155890
AG activates biosynthesis of gibberellin, which has been proposed to promote the shift from meristem identity to differentiation PMID: 15634696
AG and miR172 have distinct functions in flower development and that they largely act independently in the negative regulation of AP2. PMID: 17573799
These results indicate that stamen identity and differentiation control by AG is achieved by the regulation of different transcriptional cascades in different floral stages. PMID: 17981996
Transgenic Arabidopsis plants harboring two tissue-specific promoters, fAGIP and rAGIP in forward and reverse orientations, fAGIP::GUS or rAGIP::GUS displayed similar GUS expression specifically in carpel and stamen tissues and their primordial cells. PMID: 18256838
PERIANTHIA (PAN) plays a role in regulating stem cell fate by directly controlling AG expression and suggest that this activity is spatially restricted to the centermost region of the AG expression domain. PMID: 19395638
Upon enhanced central AG expression, the C-domain laterally expands necessitating tuning of the expression level of C-function genes in the wild type. PMID: 19554348

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Subcellular Location

Nucleus.

Tissue Specificity

Detected early in the floral meristem but mostly expressed in stamen and carpel primordia.

Database Links

KEGG: ath:AT4G18960

STRING: 3702.AT4G18960.1

UniGene: At.28088

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