AGL27 Antibody

Code CSB-PA858336XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGL27 Polyclonal antibody
Uniprot No.
Target Names
AGL27
Alternative Names
AGL27 antibody; FK1 antibody; FLM antibody; MAF1 antibody; At1g77080 antibody; F22K20.15 antibody; Agamous-like MADS-box protein AGL27 antibody; MADS box FLC1-like nuclear protein antibody; Protein FLOWERING LOCUS M antibody; Protein MADS AFFECTING FLOWERING 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AGL27 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Probable transcription factor involved in the negative regulation of flowering time in both long and short days, probably through the photoperiodic and vernalization pathways. Prevents premature flowering.
Gene References into Functions
  1. The data support the role of FLM-beta as a flower repressor and provide evidence that a contribution of FLM-delta to the regulation of flowering time in wild-type Arabidopsis thaliana seems unlikely. PMID: 29036339
  2. This study's findings suggest a model in which the loss of the floral repressor FLM occurs through mRNA degradation in response to elevated temperatures, signifying a role for alternative splicing coupled with nonsense-mediated mRNA decay (AS-NMD) in conferring environmental responses in plants. PMID: 27243649
  3. we identify insertion polymorphisms in the first intron of FLM as causative for accelerated flowering in many natural A. thaliana accessions, especially in cool (15 degrees C) temperatures PMID: 26492483
  4. FLM and MAF2 repress flowering in parallel at low temperature. PMID: 25955034
  5. SVP-FLM-beta complex is predominately formed at low temperatures and prevents precocious flowering; by contrast, the competing SVP-FLM-delta complex is impaired in DNA binding and acts as a dominant-negative activator of flowering at higher temperatures PMID: 24067612

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in most plant tissues, embryo, seedlings, roots, leaves, stems, inflorescence, pollen, siliques and flowers.
Database Links

KEGG: ath:AT1G77080

STRING: 3702.AT1G77080.4

UniGene: At.19799

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