Product Details

Full Product Name

Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ago1 Polyclonal antibody

Uniprot No.

O74957

Target Names

ago1

Alternative Names

ago1 antibody; csp9 antibody; SPCC736.11Protein argonaute antibody; Cell cycle control protein ago1 antibody; Eukaryotic translation initiation factor 2C 2-like protein ago1 antibody; PAZ Piwi domain protein ago1 antibody; Protein slicer antibody; RNA interference pathway protein ago1 antibody

Raised in

Rabbit

Species Reactivity

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Immunogen

Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ago1 protein

Immunogen Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required for G1 arrest and mating in response to nitrogen starvation. Ago1 regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. Required, indirectly, for regulated hyperphosphorylation of cdc2. Has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation, accurate chromosome segregation, centromere cohesion and telomere function during mitosis and meiosis. Required for silencing at the centromeres and for initiation of transcriptionally silent heterochromatin at the mating type locus. Promotes histone H3K9 methylation necessary for centromere function. Required for recruitment of swi6 and cohesin to an ectopic dg repeat. A member of the RNA-induced transcriptional silencing (RITS) complex which is involved in the biosynthesis of dsRNA from primer siRNAs provided by the RNA-directed RNA polymerase (RDRC) complex. Has ribonuclease H-like cleavage (slicing) activity towards target messages complementary to siRNA and can direct site-specific cleavage of RNA substrates via siRNA. Slicing activity is required for both post-transcriptional and transcriptional gene silencing as well as for histone H3 'Lys-10' methylation spreading, conversion of double-stranded siRNA to single-stranded siRNA and siRNA-dependent association of ago1 with chromatin. A member of the argonaute siRNA chaperone (ARC) complex which is required for histone H3K9 methylation, heterochromatin assembly and siRNA generation. The ARC complex contains mostly double-stranded siRNA.

Gene References into Functions

Data indicate that surveillance of Argonaute-bound small RNAs by Cid14/Cid16 and the exosome protects the genome from uncontrolled RNAi and reveal a rapid RNAi-based adaptation to stress conditions. PMID: 28541282
Survival in quiescence requires the euchromatic deployment of Clr4 by Ago1-associated small RNAs. PMID: 27984744
The Slicer activity of fission yeast Ago1 can be bypassed in sRNA-mediated H3K9 methylation, dsRNA synthesis, siRNA amplification, and gene silencing. PMID: 27397687
Nuclear accumulation of poly(A)(+) RNAs was detected in mutants of ago1(+) and ptr1(+), suggesting there is a functional link between the cell cycle checkpoint and RNAi-mediated RNA quality control. PMID: 22989756
Argonaute requires the conserved aspartate-aspartate-histidine motif for heterochromatic silencing & for ribonuclease H-like cleavage (slicing) of target messages complementary to siRNA; spreading requires read-through transcription & slicing by Argonaute PMID: 16931764
the amino terminus of Ago1 binds to proteins that function in cell cycle regulation including 14-3-3 proteins PMID: 17043360
Results describe a repetitive motif within Tas3, termed the 'Argonaute hook', that is conserved from yeast to humans and binds Ago1 and 2 through their PIWI domains in vitro and in vivo. PMID: 17891150

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Subcellular Location

Cytoplasm. Nucleus. Chromosome, centromere. Chromosome, telomere. Note=Associates with telomeric and mating-type region heterochromatin.

Protein Families

Argonaute family, Ago subfamily

Database Links

KEGG: spo:SPCC736.11

STRING: 4896.SPCC736.11.1

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ago1 Antibody

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