Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) AGO3 Polyclonal antibody

Uniprot No.

Q7PLK0

Target Names

AGO3

Alternative Names

AGO3 antibody; CG40300 antibody; Protein argonaute-3 antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) AGO3 protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Acts via the piwi-interacting RNA (piRNA) metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates predominantly with sense piRNAs that contain adenine at nucleotide 10, but shows no preference for uridine at the 5' end. In testis, however, associates with Su(Ste) and AT-chX-1 piRNAs mostly produced from antisense precursors. Shows RNA cleavage activity. In the germline, acts to amplify pools of antisense piRNAs, among others Su(Ste), AT-chX-1 and roo, and to limit sense piRNA accumulation. Forms a complex with smg, twin, aub and specific piRNAs that targets nos mRNA (and probably other maternal mRNAS) for deadenylation promoting its decay during early embryogenesis. Involved in transposon silencing in the adult brain.

Gene References into Functions

Although Argonaute3 slicing is required to efficiently trigger phased piRNA production, an alternative, slicing-independent pathway suffices to generate Piwi-bound piRNAs that repress transcription of a subset of transposon families. PMID: 26340424
AGO3 Slicer activity is essential for piRNA amplification. PMID: 25049272
Biogenesis pathways of piRNAs loaded onto AGO3 in testes. PMID: 20980675
PIWI subfamily member AGO3 had Slicer activity in vitro; data support model in which formation of a 5' terminus within rasiRNA precursors is guided by rasiRNAs originating from transcripts of the other strand in concert with Slicer activity of PIWI PMID: 17322028
Study examined piwi-interacting RNAs (piRNAs) associated with each Drosophila Piwi protein and found that Piwi and Aubergine bind predominantly RNAs antisense to transposons, whereas Ago3 complexes contain predominantly sense piRNAs. PMID: 17346786
Ago3 acts to amplify piRNA pools and to enforce on them an antisense bias, increasing the number of piRNAs that can act to silence transposons PMID: 19395009

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Argonaute family, Piwi subfamily

Tissue Specificity

In ovary, expressed in germline stem cells, germline cyst cells, nurse cells and oocytes during early stages. Also found in the somatic cap cells of the germarium. In testis, expressed in germline stem cells, primary gonial cells and early spermatocytes.

Database Links

KEGG: dme:Dmel_CG40300

STRING: 7227.FBpp0289159

UniGene: Dm.33791

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AGO3 Antibody

Product Details

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Target Background