AGO7 Antibody

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Code	CSB-PA866383XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO7 Polyclonal antibody

Uniprot No.

Q9C793

Target Names

AGO7

Alternative Names

AGO7 antibody; ZIP antibody; At1g69440 antibody; F10D13.11Protein argonaute 7 antibody; Protein ZIPPY antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AGO7 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for the processing of 21 nucleotide trans-acting siRNAs (ta-siRNAs) derived from TAS3a transcripts. Associates preferentially with the microRNA (miRNA) miR390 which guides the cleavage of TAS3 precursor RNA. Seems to act as miR390 specific slicer. Associates mainly with small RNAs of 21 nucleotide in length and with a 5' terminal adenosine. Acts in the RDR6/SGS3/DCL4/AGO7 trans-acting siRNA pathway involved in leaf developmental timing. Does not seems to act on leaf polarity. Required for the production of the 30-40nt bacterial-induced long siRNAs (lsiRNA). Involved in antiviral RNA silencing by contributing to efficient viral RNAs clearance. Targets less structured viral RNAs than AGO1 which is capable of targeting RNAs with more compact structures.

Gene References into Functions

Arabidopsis ARGONAUTE7 selects microRNA390 through multiple checkpoints during RISC assembly. PMID: 23732541
It was shown that AGO7 is needed in the cytoplasm and membranous siRNA bodies for the processing of the TAS3 precursor, showing a hitherto unknown role for membrane-associated ribonucleoparticles in trans-acting-siRNA biogenesis and AGO action in plants. PMID: 22327216
Study shows that miR390-ARGONAUTE7 complexes function in distinct cleavage or noncleavage modes at two target sites in TAS3a transcripts. PMID: 18342362

Protein Families

Argonaute family, Ago subfamily

Tissue Specificity

Expressed in leaves and floral buds, and at low levels in roots.

Database Links

KEGG: ath:AT1G69440

STRING: 3702.AT1G69440.1

UniGene: At.35421

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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AGO7 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background