AHK1 Antibody

Code CSB-PA890472XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AHK1 Polyclonal antibody
Uniprot No.
Target Names
AHK1
Alternative Names
AHK1 antibody; At2g17820 antibody; T13L16.16 antibody; T17A5Histidine kinase 1 antibody; EC 2.7.13.3 antibody; Arabidopsis histidine kinase 1 antibody; AtHK1 antibody; Protein AUTHENTIC HIS-KINASE 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AHK1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Functions as an osmosensor histidine kinase that detects water stress and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Positive regulator of drought and salt stress responses, and abscisic acid (ABA) signaling. Confers drought tolerance, probably by regulating levels of ABA accumulation. Plays a redundant role in regulating plant growth and development. Required for the regulation of desiccation processes during seed formation.
Gene References into Functions
  1. results show that de novo biosynthesis of abscisic acid, via up-regulation of the rate-limiting NCED3 gene, can be triggered by changes in leaf turgor; AHK1 does not function as the critical turgor-sensing receptor PMID: 28449122
  2. Data indicate that the unimpaired growth, abscisic acid (ABA), proline, and solute accumulation of ahk1 mutants at low psi(w) suggest that AHK1 may not be the main plant osmosensor required for low water potential psi(w) tolerance. PMID: 23184230
  3. AHK1 plays important roles in plant growth because the ahk1 ahk2 ahk3 triple mutant showed further reduced growth. PMID: 18077346
  4. Since overexpression of ATHK1 results in increased water stress tolerance, our observations suggest a new top-down route to increasing drought resistance via receptor-mediated increases in sensing water status PMID: 18441212
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Tissue Specificity
Mostly expressed in roots, and, to a lower extent, in stems, leaves and flowers.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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