AHK2 Antibody

Code CSB-PA863209XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AHK2 Polyclonal antibody
Uniprot No.
Target Names
AHK2
Alternative Names
AHK2 antibody; At5g35750 antibody; MXH1.16Histidine kinase 2 antibody; EC 2.7.13.3 antibody; Arabidopsis histidine kinase 2 antibody; AtHK2 antibody; Protein AUTHENTIC HIS-KINASE 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AHK2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Cytokinins (CK) receptor related to bacterial two-component regulators. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. In the presence of cytokinin, feeds phosphate to phosphorelay-integrating histidine phosphotransfer protein (HPt) and activates subsequent cascade. Involved in meristems establishment in seedlings. Redundant negative regulator of drought and salt stress responses and abscisic acid (ABA) signaling. Together with AHK3, plays a negative regulatory role in cold stress signaling via inhibition of ABA response, occurring independently of the cold acclimation pathway. Redundant positive regulator of cytokinin signaling that regulates many developmental processes including seed germination, cell division, seed size, chlorophyll retention during leaf senescence, root repression and shoot promotion. Involved in alkamides (e.g. N-isobutyl decanamide) and N-acylethanolamides (NAE) signaling that control meristematic activity and differentiation processes during plant development. Contributes to vascular bundle formation and secondary growth in a cytokinin-dependent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Together with AHK4, required for growth and reproduction promotion stimulated by the endophytic fungus Piriformospora indica in a trans-zeatin-dependent manner. Required by the cytokinin-dependent flower development regulation pathway.
Gene References into Functions
  1. Data show that the gain-of-function alleles of cytokinin receptors AHK2 and AHK3 have an impact on cytokinin homeostasis and lower the cytokinin content, suggesting a feedback regulation of cytokinin metabolism by the cytokinin signaling pathway. PMID: 28096190
  2. Data indicate that accumulation of transcripts of several plastid-encoded genes is dependent on the capital A, Cyrilliccapital EN, Cyrilliccapital KA, Cyrillic2/capital A, Cyrilliccapital EN, Cyrilliccapital KA, Cyrillic3 receptor combination. PMID: 28150126
  3. The AHK2/AHK3-Dependent Two-Component Signaling System Causes Ser/Thr/Tyr Phosphorylation and Dephosphorylation in Arabidopsis thaliana PMID: 26485051
  4. The role of the histidine kinases AHK2 and AHK3 in light- and abscisic acid-mediated stomatal aperture control. PMID: 24758561
  5. the protective function of cytokinin during light stress depends on the Arabidopsis histidine KINASE2 (AHK2) and AHK3 receptors. PMID: 24424319

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue Specificity
Expressed in roots, leaves and flowers, mostly in the vascular tissues. Present in seedlings.
Database Links

KEGG: ath:AT5G35750

STRING: 3702.AT5G35750.1

UniGene: At.23233

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