Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AHK5 Polyclonal antibody

Uniprot No.

Q3S4A7

Target Names

AHK5

Alternative Names

AHK5 antibody; CKI2 antibody; At5g10720 antibody; MAJ23.80Histidine kinase 5 antibody; EC 2.7.13.3 antibody; Arabidopsis histidine kinase 5 antibody; AtHK5 antibody; Protein AUTHENTIC HIS-KINASE 5 antibody; Protein CYTOKININ-INDEPENDENT 2 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AHK5 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Negative regulator of the ETR1-dependent abscisic acid (ABA) and ethylene signaling pathway that inhibits the root elongation. Promotes stomatal closure. Regulates stomatal opening by integrating multiple signals via hydrogen peroxide H(2)O(2) homeostasis in guard cells in an ABA-independent manner. May contribute to basal defense mechanisms by closing stomata in the presence of bacterial pathogens. Regulates both hormone levels and ROS production in response to stress. Required for full immunity to bacterial pathogen and necrotrophic fungus.

Gene References into Functions

data suggest the presence of a complex TCS signaling network comprising of at least AHK5, several AHPs and response regulators, which modulate stomatal closure in response to HO and ethylene. PMID: 22951399
A model which proposes a role for reactive oxygen species (ROS) and hormones in integrating abiotic and biotic stress responses via AHK5, is proposed. PMID: 22827948
This is the first report of a role for AHK5 in the regulation of survival following challenge by a hemi-biotrophic bacterium and a necrotrophic fungus, as well as in the growth response to salt stress. PMID: 22256998
findings identify an integral signalling function for AHK5 that acts to integrate multiple signals via H(2)O(2) homeostasis and is independent of ABA signalling in guard cells PMID: 18560512

Subcellular Location

Cell membrane; Peripheral membrane protein. Cytoplasm.

Tissue Specificity

Present in light-grown but not in etiolated seedlings. Mostly expressed in roots flowers and siliques, and, to a lower extent, in stems and leaves, especially in guard cells.

Database Links

KEGG: ath:AT5G10720

STRING: 3702.AT5G10720.1

UniGene: At.19135

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AHK5 Antibody

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