AHP1 Antibody

Code CSB-PA992114XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AHP1 Polyclonal antibody
Uniprot No.
Target Names
AHP1
Alternative Names
AHP1 antibody; ATHP3 antibody; At3g21510 antibody; MIL23.8 antibody; Histidine-containing phosphotransfer protein 1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AHP1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Functions as two-component phosphorelay mediators between cytokinin sensor histidine kinases and response regulators (B-type ARRs). Plays an important role in propagating cytokinin signal transduction through the multistep His-to-Asp phosphorelay.
Gene References into Functions
  1. Mutating selected interface residues of ARR4 either abolished or destabilized the interaction. D45A and Y96A mutations weakened interaction with AHP1, and exhibited weaker rescue of root elongation in the hextuple mutants. PMID: 25643735
  2. The first crystal structure of a plant histidine kinase receiver domain(RD), AHK5(RD), in complex with one of its cognate phosphotransfer proteins, AHP1. PMID: 23132142
  3. interaction of ETR1 and AHP1 PMID: 21912672
  4. Fluorescence polarization studies demonstrate a specific interaction of ETR1 with the histidine-containing transfer protein AHP1, supporting the idea that a phosphorelay module is involved in ethylene signaling PMID: 18384742
Subcellular Location
Cytoplasm, cytosol. Nucleus.
Tissue Specificity
Strongly expressed in roots.
Database Links

KEGG: ath:AT3G21510

STRING: 3702.AT3G21510.1

UniGene: At.24157

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