Product Details

Full Product Name

Rabbit anti-Caenorhabditis elegans air-2 Polyclonal antibody

Uniprot No.

O01427

Target Names

air-2

Alternative Names

air-2 antibody; stu-7 antibody; B0207.4Aurora/IPL1-related protein kinase 2 antibody; EC 2.7.11.1 antibody; Serine/threonine-protein kinase aurora-B antibody

Raised in

Rabbit

Species Reactivity

Caenorhabditis elegans

Immunogen

Recombinant Caenorhabditis elegans air-2 protein

Immunogen Species

Caenorhabditis elegans

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of chromosome segregation and cytokinesis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation. Required for histone H3 phosphorylation during segregation of homologous chromosomes in meiosis and mitosis. Required for histone H3 'Ser-10' phosphorylation. Phosphorylates tlk-1 at 'Ser-634', which enhances its activity. Phosphorylates zen-4 at 'Ser-680'(PubMed:15854913). Required for the recruitment of bub-1 to the ring-shaped domain between chromosomes during meiotic anaphase I. Also required for the localization of the condensin I complex subunit smc-4 to mitotic chromosomes. Acts at the spindle midzone and the midbody to prevent cleavage furrow regression upon chromatin obstructions during cytokinesis.

Gene References into Functions

Spatiotemporal regulation of Aurora B recruitment ensures release of cohesion during C. elegans oocyte meiosis. PMID: 29483514
Chromatin obstructions activated Aurora B at the spindle midzone, which is needed for the abscission checkpoint in other systems. Condensin I, but not condensin II, localizes to the spindle midzone in anaphase and to the midbody during normal cytokinesis. PMID: 23684975
LAB-1 directly targets GSP-2 to the chromatin during meiotic onset, thus restricting aberrant AIR-2 accumulation at that stage and promoting normal establishment and maintenance of sister chromatid cohesion. PMID: 22927794
The results suggest that CDC-48s control the restricted localization of AIR-2 to the cohesion sites of homologous chromatids in meiosis I. PMID: 22735043
During both mitosis and meiosis, the localization of condensin I, but not condensin II, parallels the localization of air2. PMID: 22025633
TLK-1 functions in cytokinesis by localizing AIR-2 to the midzone microtubules. PMID: 20705056
air-2 phosphorylates and is required for the localization of a bimc kinase to meiotic and mitotic spindles. PMID: 15548597
An Afg2/Spaf-related Cdc48-like AAA ATPase regulates the stability and activity of the C. elegans Aurora B kinase AIR-2. PMID: 18854144

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Subcellular Location

Cytoplasm, cytoskeleton. Chromosome. Midbody. Cytoplasm, cytoskeleton, spindle.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily

Database Links

KEGG: cel:CELE_B0207.4

STRING: 6239.B0207.4

UniGene: Cel.6613

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air-2 Antibody

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