akt-1 Antibody

Code CSB-PA621037XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans akt-1 Polyclonal antibody
Uniprot No.
Target Names
akt-1
Alternative Names
akt-1 antibody; C12D8.10Serine/threonine-protein kinase akt-1 antibody; EC 2.7.11.1 antibody; Protein kinase B akt-1 antibody; PKB akt-1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans akt-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Acts downstream of PI3 kinase age-1 and kinase pdk-1 in the daf-2/insulin receptor-like transduction pathway. Phosphorylates Forkhead-related daf-16 and the longevity-promoting skn-1 transcription factors, which inhibits their entry into the nucleus and antagonizes their functions. Has an essential role in regulating developmental arrest at the dauer stage. Plays a role in immune function and pathogen resistance. Regulates salt chemotaxis learning. Downstream of age-1 and together with akt-2 and sgk-1, promotes cell survival during embryonic development.
Gene References into Functions
  1. this study shows that akt-1 and akt-2 negatively regulate DNA-damage-induced apoptosis in the C. elegans germline and the antiapoptotic activity of akt-1 is independent of its target gene daf-16 but dependent on cep-1/p53. PMID: 17276923
  2. Modulation of pptr-1 affects insulin/IGF-1 signaling pathway-associated phenotypes including life span, dauer, stress resistance, and fat storage; study shows that PPTR-1 functions by regulating worm AKT-1 phosphorylation at Thr 350. PMID: 19249087
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, RAC subfamily
Tissue Specificity
Expressed in neurons, muscle cells of the pharynx, rectal gland cells, vulva and spermatheca.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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