Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ALD1 Polyclonal antibody

Uniprot No.

Q9ZQI7

Target Names

ALD1

Alternative Names

ALD1 antibody; At2g13810 antibody; F13J11.16Aminotransferase ALD1 antibody; chloroplastic antibody; EC 2.6.1.- antibody; AGD2-like defense response protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ALD1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Aminotransferase involved in local and systemic acquired resistance (SAR) to the bacterial pathogen P.syringae. Required for salicylic acid (SA) and camalexin accumulation upon pathogen infection. Possesses aminotransferase activity in vitro and may generate amino-acid-derived defense signals in vivo. May be involved in ethylene-induced senescence signaling. Involved in the biosynthesis of pipecolate (Pip), a metabolite that orchestrates defense amplification, positive regulation of SA biosynthesis, and priming to guarantee effective local resistance induction and the establishment of SAR. Converts lysine to alpha-keto-epsilon-aminocaproate, which then can spontaneously cyclize to form delta-(1)-piperideine-2-carboxylate (P2C). P2C is converted to Pip by SARD4. May produce non-Pip metabolites that play roles in immunity. Involved in the synthesis of distinct metabolite signals that affect basal and early defenses, and later defense responses.

Gene References into Functions

The structure of ALD1 from the flowering plant Arabidopsis thaliana (AtALD1) was solved at a resolution of 2.3 A. PMID: 23385743
ALD1 plays important roles in edr2-mediated defense responses, and senescence and revealed a crosstalk between ethylene and salicylic acid signaling mediated by ALD1 and EDR2. PMID: 21530897
We now find agd2-like defense response protein1 to be a key defense component that acts in multiple contexts and partially requires the PHYTOALEXIN DEFICIENT4 (PAD4) defense regulatory gene for its expression in response to infection. [ALD1] PMID: 15447647
ALD1 is partially required for Pseudomonas syringae HopW1-1-induced disease resistance. PMID: 18266921

Subcellular Location

Plastid, chloroplast.

Protein Families

Class-I pyridoxal-phosphate-dependent aminotransferase family, LL-diaminopimelate aminotransferase subfamily

Tissue Specificity

Highly expressed in senescing leaves, flowers, siliques and seeds.

Database Links

KEGG: ath:AT2G13810

STRING: 3702.AT2G13810.1

UniGene: At.26317

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ALD1 Antibody

Product Details

Customer Reviews and Q&A

Target Background