Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ALE1 Polyclonal antibody

Uniprot No.

Q08548

Target Names

ALE1

Alternative Names

ALE1 antibody; LCA1 antibody; LPT1 antibody; SLC4 antibody; YOR175C antibody; O3635 antibody; Lysophospholipid acyltransferase antibody; LPLAT antibody; EC 2.3.1.23 antibody; EC 2.3.1.51 antibody; 1-acyl-sn-glycerol-3-phosphate acyltransferase antibody; AGPAT antibody; Lysophosphatidic acid acyltransferase antibody; LPAAT antibody; Lysophosphatidylcholine acyltransferase antibody; LPCAT antibody; Lysophosphatidylethanolamine acyltransferase antibody; LPEAT antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ALE1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Broad specificity membrane-bound O-acyltransferase that mediates the incorporation of unsaturated acyl chains into the sn-2 position of various lysophospholipids. Preferentially acylates lysophosphocholine (LPC), but also lysophosphoethanolamine (LPE), lysophosphatidylglycerol (LPG), lysophosphatidic acid (LPA), lysophosphoethanolamine (LPE), lysophosphoinositol (LPI), and lysophosphoserine (LPS). Prefers an acyl residue to an alkyl residue at the sn-1 position of lysophospholipid acceptors. Accepts acyl chains in acyl-CoA from C-2 to C-20, and shows strong preference for unsaturated acyl-CoAs with 16-20 carbons. Together with SLC1, plays a central role in phosphatidic acid (PA) biosynthesis. PA is the intermediate, from which all glycerophospholipids are synthesized. Can also introduce an acyl chain at the sn-1 position of the lysophosphatidylcholine analog 1-hydroxy-2-hexadecyl-sn-glycero-3-phosphocholine (HHPC).

Gene References into Functions

D146 and E297 act in conjunction with H382 as nucleophiles that attack the hydroxyl group in lysophospholipids in a general acid/base mechanism in Lpt1. PMID: 26382650
that a previously uncharacterized open reading frame, YOR175C, encodes the major acyl-CoA-dependent lysophosphatidylethanolamine acyltransferase activity in yeast PMID: 17652094
Identification of LCA1 provides an essential molecular tool for further study of Lands cycle in phosphatidylcholine turnover PMID: 17996202

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Membrane-bound acyltransferase family

Database Links

KEGG: sce:YOR175C

STRING: 4932.YOR175C

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ALE1 Antibody

Product Details

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Target Background