wol Antibody

Code CSB-PA219177XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) wol Polyclonal antibody
Uniprot No.
Target Names
wol
Alternative Names
wol antibody; alg5 antibody; CG7870Dolichyl-phosphate beta-glucosyltransferase antibody; EC 2.4.1.117 antibody; Wollknaeuel antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) wol protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for normal production of N-glycosylated proteins in the endoplasmic reticulum (ER). Required for embryonic segmentation, dorsal-ventral patterning and gastrulation. Required for chitin orientation and shaping of the apical and lateral plasma membranes of epidermal cells during cuticle differentiation. Also required for correctly shaping apical membrane topology of the epithelia of other organs such as the midgut and the hindgut.
Gene References into Functions
  1. Mutations in the Drosophila alg5 gene that codes for an enzyme initiating the glucosylation of the dolichol-linked oligosaccharide decrease, glucosylation and the amounts of N-glycosylated proteins such as the cuticle-organizing factor Knickkopf. PMID: 21199819
  2. A cDNA from Drosophila ALG5, also known as wollknauel (wol), is able to partially complement the hypoglycosylation phenotype of alg5 mutant S. cerevisiae, whereas the two wol mutant alleles failed to complement. PMID: 18403407
Subcellular Location
Endoplasmic reticulum membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 2 family
Database Links

KEGG: dme:Dmel_CG7870

STRING: 7227.FBpp0079234

UniGene: Dm.11591

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