xit Antibody

Code CSB-PA894774XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) xit Polyclonal antibody
Uniprot No.
Target Names
xit
Alternative Names
xit antibody; CG4542Probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase antibody; Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase antibody; EC 2.4.1.265 antibody; Asparagine-linked glycosylation protein 8 homolog antibody; Dol-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichyl alpha-1,3-glucosyltransferase antibody; Protein xiantuan antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) xit protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(1)Man(9)GlcNAc(2)-PP-Dol. Functions in developmental processes such as germband extension, the apical constriction of mesoderm precursor cells and ventral furrow formation in early embryogenesis prior to gastrulation. Involved in the glycosylation and intracellular distribution of shg (E-cadherin). Function in cell intercalation in the lateral epidermis during germband extension may be due to its effect on shg.
Gene References into Functions
  1. The glucosyltransferase Xiantuan (xit, CG4542) of the endoplasmic reticulum specifically affects E-Cadherin expression and is required for gastrulation movements in Drosophila. PMID: 24681004
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
ALG6/ALG8 glucosyltransferase family
Database Links

KEGG: dme:Dmel_CG4542

STRING: 7227.FBpp0070931

UniGene: Dm.59

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