pepN Antibody

Code CSB-PA001827XA01ENV
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Product Details

Full Product Name
Rabbit anti-Escherichia coli (strain K12) pepN Polyclonal antibody
Uniprot No.
Target Names
pepN
Alternative Names
pepN antibody; b0932 antibody; JW0915Aminopeptidase N antibody; EC 3.4.11.2 antibody; Alpha-aminoacylpeptide hydrolase antibody
Raised in
Rabbit
Species Reactivity
Escherichia coli (strain K12)
Immunogen
Recombinant Escherichia coli (strain K12) pepN protein
Immunogen Species
Escherichia coli (strain K12)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Aminopeptidase N is involved in the degradation of intracellular peptides generated by protein breakdown during normal growth as well as in response to nutrient starvation.
Gene References into Functions
  1. Here we describe the crystal structure of Escherichia coli aminopeptidase N (ePepN), a model protein of the M1 family aminopeptidases in complex with actinonin PMID: 25644575
  2. Importantly, growth profiles of DeltapepN upon expression of wild-type or mutant proteins demonstrated the importance of catalytic activity during nutritional downshift and high temperature but not sodium salicylate-induced stress. PMID: 22766257
  3. structures of the ligand-free form and the enzyme-bestatin complex were determined at 1.5- and 1.6-A resolution, respectively PMID: 16885166
  4. Data show that PepN can effectively process heptapeptide-nucleotide microcin C (McC). PMID: 18223070
Subcellular Location
Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Peptidase M1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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