AMS Antibody

Datasheet

Code	CSB-PA932265XA01DOA
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AMS Polyclonal antibody

Uniprot No.

Q9ZVX2

Target Names

AMS

Alternative Names

AMS antibody; BHLH21 antibody; EN48 antibody; At2g16910 antibody; F12A24.9Transcription factor ABORTED MICROSPORES antibody; Basic helix-loop-helix protein 21 antibody; AtbHLH21 antibody; bHLH 21 antibody; Transcription factor EN 48 antibody; bHLH transcription factor bHLH021 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AMS protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Transcription factor. Plays a crucial role in tapetum development. Required for male fertility and pollen differentiation, especially during the post-meiotic transcriptional regulation of microspore development within the developing anther. Binds E-box regions in the AHL16/TEK promoter.

Gene References into Functions

The TDF1-AMS complex acts in concert to activate EXPB5 expression through a feed-forward loop. The identification of the regulatory pathway between TDF1 and AMS provides an interlocked feed-forward loop circuit that precisely regulates the transcriptional cascades that support anther development. PMID: 28940573
AMS may competitively form a protein complex with other tapetum-expressed transcription factors, and that biphasic regulation is due to repression of upstream regulators and promotion of AMS protein degradation. PMID: 27787905
AMS mutation affects genes with putative functions in phosphorylation, exocytosis, stress-response and ubiquitin-proteasome pathways during male reproduction. PMID: 22336428
DYT1, TDF1 and AMS function in early tapetum development, while MS188 and MS1 are important for late tapetum development. PMID: 21957980
AMS alters the expression of genes required for tapetal differentiation and degradation. PMID: 20118226
AMS can target ASHR3 to chromatin and regulate genes involved in stamen development and function PMID: 17978851

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Subcellular Location

Nucleus.

Tissue Specificity

Mostly expressed in closed, post-meiotic buds, and, to a lower extent, in pre-meiotic buds. Detected in leaves, stems, and flowers.

Database Links

KEGG: ath:AT2G16910

STRING: 3702.AT2G16910.1

UniGene: At.48482

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Service

Phage Display Service

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Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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