AMT1-1 Antibody

Datasheet

Code	CSB-PA345359XA01DOA
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Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AMT1-1 Polyclonal antibody

Uniprot No.

P54144

Target Names

AMT1-1

Alternative Names

AMT1-1 antibody; At4g13510 antibody; T6G15.60Ammonium transporter 1 member 1 antibody; AtAMT1;1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AMT1-1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

High affinity ammonium transporter probably involved in ammonium uptake from the soil, long-distance transport to the shoots and re-uptake of apoplastic ammonium that derives from photorespiration in shoots. Contributes with AMT1-3 to the overall ammonium uptake capacity in roots under nitrogen-deficiency conditions.

Gene References into Functions

AtNRT2.1 expression plays a key role in the regulation of AtAMT1.1 expression and in the NH(4)(+) ion influx, differentiating the nitrogen source, and particularly, the lack of it. PMID: 22137605
Electrophysiological studies of Arabidopsis AtAMT1;1 expressed in oocytes revealed passive, Deltapsi-driven transport of NH(4)(+) through this protein. PMID: 16806203
participates in concentrative NH4+ acquisition in roots, in long-distance transport to the shoots, and in re-uptake of apoplastic NH4+ that derives from photorespiration in shoots PMID: 16917981
The posttranscriptional regulation of AMT1 in transgenic N. tabacum plants is reported. PMID: 17172286
the soluble cytosolic carboxy terminus of AMT1 from Arabidopsis thaliana serves as an allosteric regulator essential for function; mutations in the C-terminal domain led to loss of transport activity PMID: 17293878

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Ammonia transporter channel (TC 1.A.11.2) family

Tissue Specificity

Highly expressed in roots. Expressed in root tips, root hairs, root epidermis, rhizodermis, cortex and pericycle. Expressed in leaves epidermal and mesophyll cells.

Database Links

KEGG: ath:AT4G13510

STRING: 3702.AT4G13510.1

UniGene: At.23790

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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AMT1-1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background