lem-3 Antibody

Code CSB-PA799930XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans lem-3 Polyclonal antibody
Uniprot No.
Target Names
lem-3
Alternative Names
lem-3 antibody; rad-1 antibody; F42H11.2Ankyrin repeat and LEM domain-containing protein 1 homolog antibody; EC 3.1.-.- antibody; LEM-domain containing protein 3 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans lem-3 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Endonuclease which, in association with baf-1, plays an essential role during embryogenesis in the DNA repair response following DNA damage probably by ensuring proper chromosome segregation. Also required during postembryonic cell divisions after DNA damage caused by ionizing radiation to ensure normal cell proliferation. Resolves chromatin bridges in late mitosis that result from incomplete DNA replication, defective chromosome condensation or unresolved recombination intermediates. Together with brc-1, contributes to genome integrity by resolving mitotic chromatin bridges that result from incomplete processing of DNA breaks. In parallel to the slx-1/mus-81 pathway, acts in processing early recombination intermediates in meiotic prophase I to prevent illegitimate recombination. Also involved in processing remaining, erroneous recombination intermediates that persist into the second meiotic division.
Gene References into Functions
  1. Data suggest that LEM-3 is able to process erroneous recombination intermediates that persist into the second meiotic division. PMID: 29879106
  2. LEM-3 acts at the midbody, the structure where abscission occurs at the end of cytokinesis. PMID: 29463814
  3. LEM-3 is a LEM domain containing nuclease involved in the DNA damage response in C. elegans PMID: 22383942
Subcellular Location
Cytoplasm. Nucleus. Chromosome. Midbody. Cytoplasm, cytoskeleton, spindle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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