Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) scra Polyclonal antibody

Uniprot No.

Q9V4P1

Target Names

scra

Alternative Names

scra antibody; ani antibody; CG2092Anillin antibody; Actin-binding protein 8 antibody; ABP8 antibody; Protein scraps antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) scra protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression and proper formation of the midbody. Required during cellularization of syncytial embryos for the proper formation and function of the furrow canals, the stable inward folds of the plasma membrane which separate the peripheral nuclei. Also required for the formation of the pole cells, the progenitors of the adult germline which are formed by cytokinesis of the cytoplasmic buds at the posterior pole of the syncytial embryo. Essential for embryonic viability.

Gene References into Functions

we show that the transition from the actomyosin contractile ring to the midbody ring proceeds via a previously uncharacterized maturation process that requires opposing mechanisms of removal and retention of the scaffold protein Anillin. PMID: 24217622
Anillin is required for complete closure of the contractile ring and formation of the midbody ring during cytokinesis. PMID: 22226749
the anillin pleckstrin homology domain has two functions: targeting anillin to the plasma membrane furrow by binding to phosphatidylinositol phosphate lipids to maintain furrow organization and recruiting septins to the furrow. PMID: 22197245
Data suggest that Cindr and Anillin cooperate to promote intercellular bridge stability during incomplete cytokinesis in Drosophila melanogaster. PMID: 20451383
Results show that the anillin-septin and cadherin-catenin complexes can serve as alternative cassettes to promote tight physical coupling of F-actin and myosin II to the cleavage furrow and successful completion of cytokinesis. PMID: 20237160
anillin has a role in spatially regulating the contractile activity of myosin II during cytokinesis PMID: 15496454
important role for scraps in scaffolding cleavage furrow components, directly stabilizing intracellular bridges PMID: 15930114
interaction with cleavage furrow mediates RacGAP50C's control of actomyosin ring assembly PMID: 18349071
anillin and Peanut are involved in pseudocleavage furrow ingression in syncytial embryos, a process that is regulated by Ran PMID: 18579688

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Subcellular Location

Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. Accumulates in the nucleus of newly divided cells in a diffuse staining pattern, thereby restarting the cycle.

Tissue Specificity

Accumulates in the ring canals that interconnect cells of the germline cysts in males and the ovarian follicles in females. These structures develop from arrested contractile rings after a specialized cytokinesis in which the closing of the invaginating p

Database Links

KEGG: dme:Dmel_CG2092

STRING: 7227.FBpp0087986

UniGene: Dm.508

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scra Antibody

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